Anti-Menin antibody (ab2605)

The following references may be useful to you for specific primers for positive and negative loci associated with Menin:

ab31902 and ab32963: Menin highly enriched in PCNA PRO 2, weakly enriched in PCNA PRO 1 according to PMID:18940868 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2582630/?tool=pubmed

ab2605 in ChIP: http://cancerres.aacrjournals.org/content/72/1/165.full.pdf+html

I have issued a free of charge replacement with the order number 1042072.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that typically our Abpromise guarantee holds for 6 months from when you receive the antibody,although I have made an exception in this case.Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thanks for your reply.

You are correct that lot GR21097-1 is a new lot we have in stock. We are not able to stock small sample aliqots of our antibodies unfortunately. However, since a vial of ab21873 you previously purchased is not working as expected,I can provide you with a free replacement vial of this new lot based on our Abpromise guarantee https://www.abcam.com/abpromise

If you would like to go ahead with the replacement offer, please just provide me with the PO number or Abcam order reference number associated with the purchase of ab21873, lot 937205 so I can process the replacement.

Thank you for your enquiry. I am sorry to hear of the trouble you are having with lot 937205 in ChIP. I looked in our records and there was one other complaint of a similar nature regarding ChIP with this lot of ab21873. We do have a new lot in stock now (lot GR21097-1) and I am happy to offer a replacement for the faulty vial of ab21873.

Please pass along the PO number or Abcam order reference number associated with the purchase of ab21873, lot 937205 so I can process the replacement.

Thank you for contacting us.

I have looked into whether the anti-Menin antibody (ab2605)would be suitable for the experiments you are hoping to perform. Unfortunately, the immunogen used to raise this antibody is directed against the C-terminal end of human Menin. Through epitope mapping it has been established that the antibody specifically recognises a region betweenresidue 575 and theC-terminus (residue 615) of Menin. This antibody would therefore not be suitable if you are hoping todetect the regionN-terminal of residue 575.

I have however been able to find two other antibodies which we have been raised against peptidesderived from the N-terminal sequence of Menin which may be suitable for you.Ab32963 and ab75923 are both rabbit polyclonals which have been used in Western blotting.A blocking peptide is also available for ab32963, the catalogue code for this is ab32962.

I hope this information has been of help. If you require any further information please do not hesitate to ask.

Thank you for your enquiry. The epitope recognized by ab2605 maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221). Unfortunately, the exact sequence information is proprietary. I hope this information will still be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

Thank you for your enquiry. The epitope recognized by this product maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221). Unfortunately, the exact sequence information is proprietary. If you would like an alignment done to a certain species, I can have that analyzed.

Thank for your patience. I have received a reply from the originator of ab2605. The epitope recognized by ab2605 maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221). I hope this information helps. Please don't hesitate to contact us again if you have further questions.

Thank you for your enquiry and my apologies for the delay in replying to you, I have just received further information from the lab to answer your questions. We are not sure of the exact dilution used for the image on the datasheet, it is between 1/1000 - 1/10000 and I would recommend to try a range of dilution when you do the preliminary experiments to determine optimal conditions. The secondary is a standard HRP conjugated anti rabbit antibody. The reference on the datasheet was indeed incorrect, however I found the following references which are specific for this antibody: Milne TA et al. Menin and MLL cooperatively regulate expression of cyclin-dependent kinase inhibitors. Proc Natl Acad Sci U S A 102:749-54 (2005). PubMed: 15640349 Yokoyama A et al. The menin tumor suppressor protein is an essential oncogenic cofactor for MLL-associated leukemogenesis. Cell 123:207-18 (2005). PubMed: 16239140 Hughes CM et al. Menin associates with a trithorax family histone methyltransferase complex and with the hoxc8 locus. Mol Cell 13:587-97 (2004). PubMed: 14992727 The cells used were not tranfected i.e the samples show endogenous levels of menin. Please do not hesitate to contact me if you require further information,