Recombinant Anti-MERTK antibody [EPR26358-25] (ab289636)
![Flow Cytometry - Anti-MERTK antibody [EPR26358-25] (ab289636)](https://www.abcam.com/ps/products/289/ab289636/Images/ab289636-481977-26358KO1.jpg "Flow Cytometry - Anti-MERTK antibody [EPR26358-25] (ab289636)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26358-25] to MERTK
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MERTK antibody [EPR26358-25]
See all MERTK primary antibodies -
Description
Rabbit monoclonal [EPR26358-25] to MERTK -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt: HAP1 and HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26358-25 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab289636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/500.
|
| Notes |
|---|
|
Flow Cyt
1/500. |
Target
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Function
In case of filovirus infection, seems to function as a cell entry factor. -
Tissue specificity
Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. -
Involvement in disease
Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10461 Human
- Omim: 604705 Human
- SwissProt: Q12866 Human
- Unigene: 306178 Human
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Alternative names
- c MER antibody
- c mer proto oncogene tyrosine kinase antibody
- c-mer antibody
see all
Images
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Flow Cytometry - Anti-MERTK antibody [EPR26358-25] (ab289636)
Flow cytometric analysis of Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell line, Right) / MERTK knockout HAP1 (Left) cells labeling MERTK with ab289636 at 1/500 dilution (Red) compared with Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Flow Cytometry - Anti-MERTK antibody [EPR26358-25] (ab289636)](https://www.abcam.com/ps/products/289/ab289636/Images/ab289636-481979-26358HepG2.jpg)
Flow Cytometry - Anti-MERTK antibody [EPR26358-25] (ab289636)Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell line, Left) / HepG2 (Human hepatocellular carcinoma epithelial cell line, Right) cells labeling MERTK with ab289636 at 1/500 dilution (Red) compared with Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Negative control: HeLa (PMID:32042425).
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![Anti-MERTK antibody [EPR26358-25] (ab289636)](https://www.abcam.com/ps/products/289/ab289636/Images/ab289636-482314-benefitsofrecombinantantibodies.jpg)
Anti-MERTK antibody [EPR26358-25] (ab289636)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab289636 has not yet been referenced specifically in any publications.