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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)

Reviews (1) Submit a question References (3)
Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
  • Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7738] to Methylmalonyl Coenzyme A mutase
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738]
    See all Methylmalonyl Coenzyme A mutase primary antibodies

  • Description

    Rabbit monoclonal [EPR7738] to Methylmalonyl Coenzyme A mutase

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

     

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow

  • Immunogen

    Synthetic peptide within Human Methylmalonyl Coenzyme A mutase aa 50-150 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • HeLa, K562, K-562, 293T and human fetal liver lysates; mouse and rat brain tissue lysates, human colon and kidney tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

 See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

 See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Involved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.

  • Involvement in disease

    Defects in MUT are the cause of methylmalonic aciduria type mut (MMAM) [MIM:251000]. MMAM is an often fatal disorder of organic acid metabolism. Common clinical features include lethargy, vomiting, failure to thrive, hypotonia, neurological deficit and early death. Two forms of the disease are distinguished by the presence (mut-) or absence (mut0) of residual enzyme activity. Mut0 patients have more severe neurological manifestations of the disease than do MUT- patients. MMAM is unresponsive to vitamin B12 therapy.

  • Sequence similarities

    Belongs to the methylmalonyl-CoA mutase family.
    Contains 1 B12-binding domain.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • MCM antibody
    • Methylmalonyl CoA isomerase antibody
    • Methylmalonyl CoA mutase mitochondrial antibody
    • Methylmalonyl Coenzyme A mutase antibody
    • Methylmalonyl-CoA isomerase antibody
    • Methylmalonyl-CoA mutase antibody
    • mitochondrial antibody
    • Mut antibody
    • MUTA_HUMAN antibody
    see all

Images

  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Methylmalonyl Coenzyme A mutase knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: Human liver tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab134956 (unpurified) observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab134956 was shown to specifically react with Methylmalonyl Coenzyme A mutase when Methylmalonyl Coenzyme A mutase knockout samples were used. Wild-type and Methylmalonyl Coenzyme A mutase knockout samples were subjected to SDS-PAGE. ab134956 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Methylmalonyl Coenzyme A mutase with purified ab134956 at 1:50 dilution (2.96 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956) at 1/2000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Methylmalonyl Coenzyme A mutase with ab134956 (unpurified) at 1/50 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Human fetal liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 83 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Methylmalonyl Coenzyme A mutase with ab134956 (unpurified) at dilution 1/50.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
    Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)

References (3)

Publishing research using ab134956? Please let us know so that we can cite the reference in this datasheet.

ab134956 has been referenced in 3 publications.

  • Chandler RJ  et al. Promoterless, Nuclease-Free Genome Editing Confers a Growth Advantage for Corrected Hepatocytes in Mice With Methylmalonic Acidemia. Hepatology 73:2223-2237 (2021). PubMed: 32976669
  • May FJ  et al. Central nervous system-targeted adeno-associated virus gene therapy in methylmalonic acidemia. Mol Ther Methods Clin Dev 21:765-776 (2021). PubMed: 34169115
  • Puleston DJ  et al. Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation. Cell Metab 30:352-363.e8 (2019). PubMed: 31130465

Customer reviews and Q&As

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Western blot

 Excellent
Abreviews
abreview image
Application
Western blot
Gel Running Conditions
Non-reduced Denaturing (12% gel)
Sample
Cow Tissue lysate - other (Liver)
Specification
Liver
Blocking step
No blocking step used for 1 hour(s) and 0 minute(s) · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ouattara Bazoumana

Verified customer

Submitted Jun 14 2013

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