Recombinant Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)
![Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)](https://www.abcam.com/ps/products/124/ab124807/Images/ab124807-383531-anti-mgea5oga-antibody-epr7154b-western-blot-hela-jar-293t.jpg "Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7154(B)] to MGEA5/OGA
- Suitable for: WB, IP, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MGEA5/OGA antibody [EPR7154(B)]
See all MGEA5/OGA primary antibodies -
Description
Rabbit monoclonal [EPR7154(B)] to MGEA5/OGA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MGEA5/OGA. The exact sequence is proprietary.
(Peptide available asab155521) -
Positive control
- WB: HEK-293T, HeLa, BxPC-3, JAR and fetal brain lysates. IHC-P: Human kidney and colon tissue. IP: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 9.80 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7154(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 102 kDa).
For unpurified use at 1/10000 - 1/5000. |
| IP |
1/30.
For unpurified use at 1/100 - 1/500. |
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| IHC-P |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/100 - 1/250. |
| Notes |
|---|
|
WB
1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 102 kDa). For unpurified use at 1/10000 - 1/5000. |
|
IP
1/30. For unpurified use at 1/100 - 1/500. |
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IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified use at 1/100 - 1/250. |
Target
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Function
Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1. -
Tissue specificity
Ubiquitous. Shows highest expression in the brain, placenta and pancreas. -
Sequence similarities
Belongs to the glycosyl hydrolase 84 family. -
Post-translational
modificationsProteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity. -
Cellular localization
Cytoplasm and Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10724 Human
- Omim: 604039 Human
- SwissProt: O60502 Human
- Unigene: 500842 Human
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Alternative names
- Beta-hexosaminidase antibody
- Beta-N-acetylglucosaminidase antibody
- Beta-N-acetylhexosaminidase antibody
see all
Images
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Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)All lanes : Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MGEA5 knockout HeLa cell lysate
Lane 3 : JAR cell lysate
Lane 4 : HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab124807 observed at 130 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab124807 Anti-MGEA5/OGA antibody [EPR7154(B)] was shown to specifically react with MGEA5/OGA in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265821 (knockout cell lysate ab257263) was used. Wild-type and MGEA5/OGA knockout samples were subjected to SDS-PAGE. ab124807 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)](https://www.abcam.com/ps/products/124/ab124807/Images/ab124807-337391-anti-mgea5-oga-antibody-epr7154b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling MGEA5/OGA with purified ab124807 at 1:50 dilution (12.56 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)](https://www.abcam.com/ps/products/124/ab124807/Images/ab124807-337538-anti-mgea5-oga-antibody-epr7154b-western-blot.jpg)
Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807) at 1/5000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 20ug at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)](https://www.abcam.com/ps/products/124/ab124807/Images/ab124807-337393-anti-mgea5-oga-antibody-epr7154b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)ab124807 (purified) at 1:30 dilution (2µg) immunoprecipitating MGEA5/OGA in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab124807 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124807 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)](https://www.abcam.com/ps/products/124/ab124807/Images/ab124807-138716-anti-mgea5-oga-antibody-epr7154b-western-blot.jpg)
Western blot - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)All lanes : Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807) at 1/10000 dilution
Lane 1 : HeLa lysate
Lane 2 : BxPC-3 lysate
Lane 3 : JAR lysate
Lane 4 : Fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)](https://www.abcam.com/ps/products/124/ab124807/Images/ab124807-138715-anti-mgea5-oga-antibody-epr7154b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)ab124807, at 1/100 dilution, staining MGEA5/OGA in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)](https://www.abcam.com/ps/products/124/ab124807/Images/ab124807-138714-anti-mgea5-oga-antibody-epr7154b.jpg)
OI-RD Scanning - Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807) -
![Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)](https://www.abcam.com/ps/products/124/ab124807/Images/ab124807-8-benefits-of-recombinant-antibodies.png)
Anti-MGEA5/OGA antibody [EPR7154(B)] (ab124807)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab124807 has been referenced in 22 publications.
- Sombutthaweesri T et al. Relationship Between O-GlcNAcase Expression and Prognosis of Patients With Osteosarcoma. Appl Immunohistochem Mol Morphol 30:e1-e10 (2022). PubMed: 34469899
- Wang Y et al. EGF promotes PKM2 O-GlcNAcylation by stimulating O-GlcNAc transferase phosphorylation at Y976 and their subsequent association. J Biol Chem 298:102340 (2022). PubMed: 35931120
- Cao Y et al. O-GlcNAcylation of SIRT1 Protects against Cold Stress-Induced Skeletal Muscle Damage via Amelioration of Mitochondrial Homeostasis. Int J Mol Sci 23:N/A (2022). PubMed: 36498847
- Akinbiyi EO et al. Blocked O-GlcNAc cycling alters mitochondrial morphology, function, and mass. Sci Rep 11:22106 (2021). PubMed: 34764359
- Li C et al. Hypertonic stress modulates eNOS function through O-GlcNAc modification at Thr-866. Sci Rep 11:11272 (2021). PubMed: 34050207