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RecombinantRabMAb

Recombinant Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)

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Western blot - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
  • Immunoprecipitation - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
  • Immunoprecipitation - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
  • Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24160-173] to mGluR1a - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 568 Unconjugated

Overview

  • Product name

    Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free
    See all mGluR1a primary antibodies

  • Description

    Rabbit monoclonal [EPR24160-173] to mGluR1a - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain and cerebellum tissue lysates. Rat brain and cerebellum tissue lysates. IHC-P: Mouse cerebellum and kidney tissue. Rat cerebellum and kidney tissue. IP: Mouse and rat cerebellum tissue lysate.

  • General notes

    ab289639 is the carrier free version of ab259888

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab289639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 132 kDa.
IP
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 132 kDa.
IP
Use at an assay dependent concentration.

Target

  • Function

    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.

  • Tissue specificity

    Detected in brain.

  • Involvement in disease

    Spinocerebellar ataxia, autosomal recessive, 13

  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Glutamate receptor metabotropic 1 antibody
      • GPRC1A antibody
      • GRM1 alpha antibody
      • GRM1 antibody
      • GRM1_HUMAN antibody
      • Metabotropic glutamate receptor 1 antibody
      • MGLU1 antibody
      • mGluR1 antibody
      • SCAR13 antibody
      see all

    Images

    • Western blot - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
      Western blot - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
      All lanes : Anti-mGluR1a antibody [EPR24160-173] (ab259888) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse cerebellum tissue lysate
      Lane 3 : Mouse pinnae tissue lysate
      Lane 4 : Rat brain tissue lysate
      Lane 5 : Rat cerebellum tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 132 kDa
      Observed band size: 132, 260 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This data was developed using ab259888, the same antibody clone in a different buffer formulation.

      5% NFDM/TBST was used as a blocking and diluting buffer. 

      Samples are non-boiled as boiling may cause protein aggregates.
      Low expression: pinnae (PMID: 18776920)
      The expression profile is consistent with the literature: PMID: 27721389.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)

      This data was developed using ab259888, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse cerebellum labelling mGluR1a with ab259888 at 1/2000(0.261ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse cerebellum (PMID: 30190524). The section was incubated with ab259888 (1/2000) for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunoprecipitation - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
      Immunoprecipitation - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)

      This data was developed using ab259888, the same antibody clone in a different buffer formulation.

      mGluR1a was immunoprecipated from mouse cerebellum tissue lysate 10 μg with ab259888 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab259888 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution. 

      Lane 1: (Input) Mouse cerebellum tissue lysate 10 μg

      Lane 2: Mouse cerebellum tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259888 in mouse cerebellum tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      The expression profile is consistent with the literature. PMID: 27721389.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)

      This data was developed using ab259888, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse kidney labelling mGluR1a with ab259888 at 1/2000(0.261ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Apical staining on mouse kidney. The section was incubated with ab259888 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)

      This data was developed using ab259888, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat cerebellum labelling mGluR1a with ab259888 at 1/2000(0.261ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat cerebellum. The section was incubated with ab259888 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)

      This data was developed using ab259888, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat kidney labelling mGluR1a with ab259888 at 1/2000(0.261ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Apical staining on rat kidney. The section was incubated with ab259888 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunoprecipitation - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
      Immunoprecipitation - Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)

      This data was developed using ab259888, the same antibody clone in a different buffer formulation.

      mGluR1a was immunoprecipated from rat cerebellum tissue lysate 10 μg with ab259888 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab259888 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution. 

      Lane 1: (Input) Rat cerebellum tissue lysate 10 μg

      Lane 2: Rat cerebellum tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259888 in rat cerebellum tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      The expression profile is consistent with the literature. PMID: 27721389.

    • Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)
      Anti-mGluR1a antibody [EPR24160-173] - BSA and Azide free (ab289639)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab289639? Please let us know so that we can cite the reference in this datasheet.

    ab289639 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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