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RecombinantRabMAb

Recombinant Anti-MGST1 antibody [EPR7935] (ab129175)

Reviews (1) Submit a question
Western blot - Anti-MGST1 antibody [EPR7935] (ab129175)
  • Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7935] (ab129175)
  • OI-RD Scanning - Anti-MGST1 antibody [EPR7935] (ab129175)
  • Anti-MGST1 antibody [EPR7935] (ab129175)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7935] to MGST1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-MGST1 antibody [EPR7935]
    See all MGST1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7935] to MGST1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human MGST1 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P10620

  • Positive control

    • Fetal liver lysate, HepG2 cells and cell lysate, K562 cell lysate, HT 1376 cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.

  • Tissue specificity

    Highly expressed in liver.

  • Sequence similarities

    Belongs to the MAPEG family.

  • Post-translational
    modifications

    Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.

  • Cellular localization

    Microsome. Mitochondrion outer membrane. Endoplasmic reticulum membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • MGST1 antibody
    • Glutathione S transferase 12 antibody
    • GST12 antibody
    • MGST 1 antibody
    • MGST antibody
    • MGST I antibody
    • MGST1 antibody
    • MGST1_HUMAN antibody
    • Microsomal glutathione S-transferase 1 antibody
    • Microsomal GST 1 antibody
    • Microsomal GST-1 antibody
    • Microsomal GST-I antibody
    see all

Images

  • Western blot - Anti-MGST1 antibody [EPR7935] (ab129175)
    Western blot - Anti-MGST1 antibody [EPR7935] (ab129175)
    All lanes : Anti-MGST1 antibody [EPR7935] (ab129175) at 1/1000 dilution

    Lane 1 : Fetal liver tissue lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HT 1376 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 18 kDa
    Observed band size: 16 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7935] (ab129175)
    Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7935] (ab129175)

    ab129175, at 1/100, staining MGST1 in HepG2 cells by Immunofluorescence.

  • OI-RD Scanning - Anti-MGST1 antibody [EPR7935] (ab129175)
    OI-RD Scanning - Anti-MGST1 antibody [EPR7935] (ab129175)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-MGST1 antibody [EPR7935] (ab129175)
    Anti-MGST1 antibody [EPR7935] (ab129175)

References (0)

Publishing research using ab129175? Please let us know so that we can cite the reference in this datasheet.

ab129175 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MGST1 antibody [EPR7935]

 Excellent
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE
Permeabilization
Yes - 1/5000 tween 20
Specification
Liver
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Marc Sherman

Verified customer

Submitted Dec 22 2021

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