Anti-MGST3 antibody [2G10CG7] (ab110309)
![Immunocytochemistry/ Immunofluorescence - Anti-MGST3 antibody [2G10CG7] (ab110309)](https://www.abcam.com/ps/products/110/ab110309/Images/MGST3-Primary-antibodies-ab110309-3.gif "Immunocytochemistry/ Immunofluorescence - Anti-MGST3 antibody [2G10CG7] (ab110309)")
Key features and details
- Mouse monoclonal [2G10CG7] to MGST3
- Suitable for: ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-MGST3 antibody [2G10CG7]
See all MGST3 primary antibodies -
Description
Mouse monoclonal [2G10CG7] to MGST3 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HDFn cells IP: Human and Rat liver lysates
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
The purity of ab110309 is near homogeneity as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
2G10CG7 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 2 µg/ml.
|
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
|
ICC/IF
Use a concentration of 2 µg/ml. |
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IP
Use at an assay dependent concentration. |
Target
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Function
Also functions as a glutathione peroxidase. -
Tissue specificity
Predominantly expressed in heart, skeletal muscle, and adrenal cortex. Also found in brain, placenta, liver, kidney, pancreas, thyroid, testis and ovary. Almost absent in lung, thymus and peripheral blood leukocytes. -
Sequence similarities
Belongs to the MAPEG family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 4259 Human
- Entrez Gene: 66447 Mouse
- Entrez Gene: 289197 Rat
- Omim: 604564 Human
- SwissProt: O14880 Human
- SwissProt: Q9CPU4 Mouse
- Unigene: 191734 Human
- Unigene: 218286 Mouse
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Alternative names
- Mgst3 antibody
- MGST3_HUMAN antibody
- Microsomal glutathione S transferase 3 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MGST3 antibody [2G10CG7] (ab110309)Immunocytochemistry image of ab110309-stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110309 at 2 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates primarily in the mitochondria.
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![Immunoprecipitation - Anti-MGST3 antibody [2G10CG7] (ab110309)](https://www.abcam.com/ps/products/110/ab110309/Images/MGST3-Primary-antibodies-ab110309-4.gif)
Immunoprecipitation - Anti-MGST3 antibody [2G10CG7] (ab110309)Using MitoSciences' standard immunoprecipitation protocol, ab110309 pulls down the 15kDa MGST3 protein from human and rat liver samples. HepG2 lysate and mouse liver lysate yielded smaller amounts. This identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab110309 has not yet been referenced specifically in any publications.