Name: Anti-Mib1/Mindbomb antibody [EPR2762(2)]
Brand: RabMAb
SKU: ab124929
Rating: 4 (4 reviews)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2762(2)] to Mib1/Mindbomb
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Human

Carrier Free

Product name

Anti-Mib1/Mindbomb antibody [EPR2762(2)]
See all Mib1/Mindbomb primary antibodies
Description

Rabbit monoclonal [EPR2762(2)] to Mib1/Mindbomb
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Human
Does not react with: Rat
Immunogen

Synthetic peptide within Human Mib1/Mindbomb aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- K562, HeLa, Caco2, NCCIT, and Human brain lysates; Human kidney tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.72 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2762(2)
Isotype

IgG
Research areas

Alternative Versions
- Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa membrane extract lysate (ab29547)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(3)	1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IHC-P	(1)	1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC/IF		1/100 - 1/250.

Notes
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC/IF 1/100 - 1/250.

Application notes

Is unsuitable for IP.

Function

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
Tissue specificity

Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
Pathway

Protein modification; protein ubiquitination.
Sequence similarities

Contains 9 ANK repeats.
Contains 2 MIB/HERC2 domains.
Contains 3 RING-type zinc fingers.
Contains 1 ZZ-type zinc finger.
Post-translational
modifications

Ubiquitinated. Possibly via autoubiquitination.
Cellular localization

Cytoplasm. Cell membrane. Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear.
Target information above from: UniProt accession Q86YT6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 57534 Human
- Entrez Gene: 225164 Mouse
- Omim: 608677 Human
- SwissProt: Q86YT6 Human
- SwissProt: Q80SY4 Mouse
- Unigene: 140903 Human
- Unigene: 658808 Human
- Unigene: 21500 Mouse
Alternative names
- DAPK-interacting protein 1 antibody
- Dip 1 antibody
- DIP-1 antibody
- Dip1 antibody
- E3 ubiquitin protein ligase MIB 1 antibody
- E3 ubiquitin protein ligase MIB1 antibody
- E3 ubiquitin-protein ligase mib1 antibody
- KIAA1323 antibody
- LVNC7 antibody
- MIB antibody
- mib1 antibody
- MIB1_HUMAN antibody
- Mind bomb homolog 1 antibody
- Mindbomb E3 ubiquitin protein ligase 1 antibody
- Ubiquitin ligase mind bomb antibody
- Zinc finger ZZ type with ankyrin repeat domain protein 2 antibody
- ZZANK2 antibody
- ZZZ6 antibody
see all

Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

All lanes : Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/10000 dilution

Lane 1 : Human fetal brain tissue
Lane 2 : K562 (human chronic myelogenous leukemia) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 110 kDa
Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.

Blocking and diluting buffer: 5% NFDM/TBST

This image was produced using purified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)This image is courtesy of an Abreview submitted by Mr Rudolf Jung

Immunohistochemical analysis of Human breast cancer tissue, staining Mib1/Mindbomb with ab124929.

Tissue was fixed with paraformaldehyde and permeabilized with Tween buffer for 30 minutes at 20°C; antigen retrieval was by heat mediation in Tris/EDTA buffer (pH 9). Samples were incubated with primary antibody (1/5000 in diluent) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

ab124929 staining Mib1/Mindbomb in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab124929 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

This image was produced using purified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

ab124929 staining Mib1/Mindbomb in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1: PBS in place of primary antibody.

This image was produced using purified antibody.
Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/1000 dilution + Mouse spleen tissue lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 110 kDa
Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.

Diluting and blocking buffer: 5% NFDM /TBST.

This image was produced using purified antibody.
Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/10000 dilution + Mouse brain tissue lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 110 kDa

Blocking and diluting buffer: 5% NFDM/TBST.

This image was produced using purified antibody.
Western blot - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

All lanes : Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/1000 dilution

Lane 1 : K562 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : Caco2 cell lysates
Lane 4 : NCCIT cell lysates
Lane 5 : Human brain lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 110 kDa

This image was produced using unpurified antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

ab124929, at 1/250 dilution, staining Mib1/Mindbomb in formalin-fixed, paraffin-embedded Human kidney tissue by immunohistochemistry.

This image was produced using unpurified antibody.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
OI-RD Scanning - Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D

This image was produced using unpurified antibody.
Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab124929? Please let us know so that we can cite the reference in this datasheet.

ab124929 has been referenced in 3 publications.

Majumdar U et al. Nitric oxide prevents aortic valve calcification by S-nitrosylation of USP9X to activate NOTCH signaling. Sci Adv 7:N/A (2021). PubMed: 33547080
Bauer M et al. The E3 Ubiquitin Ligase Mind Bomb 1 Controls Adenovirus Genome Release at the Nuclear Pore Complex. Cell Rep 29:3785-3795.e8 (2019). PubMed: 31851912
Izrailit J et al. Cellular stress induces TRB3/USP9x-dependent Notch activation in cancer. Oncogene N/A:N/A (2016). Human . PubMed: 27593927

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (4-12%)

Sample

Zebrafish Tissue lysate - whole (whole embryos)

Specification

whole embryos

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Evgenia Salta

Verified customer

Submitted Feb 05 2015

Application

Western blot

Loading amount

25 µg

Gel Running Conditions

Reduced Denaturing (10% gel, at RT)

Sample

Hamster Cell lysate - whole cell (CHO cella)

Specification

CHO cella

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Miss. Bassma Khamaisi

Verified customer

Submitted Feb 04 2015

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Breast)

Specification

Breast

Fixative

Paraformaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0

Permeabilization

Yes - Wash Buffer from Dako with Tween

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Rudolf Jung

Verified customer

Submitted Nov 05 2012

Application

Western blot

Sample

Human Cell lysate - whole cell (HCT116)

Loading amount

30 µg

Specification

HCT116

Gel Running Conditions

Non-reduced Denaturing (10%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 31 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Pathway

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (3)

Customer reviews and Q&As

Western blot abreview for Anti-Mib1/Mindbomb antibody [EPR2762(2)]

Western blot abreview for Anti-Mib1/Mindbomb antibody [EPR2762(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mib1/Mindbomb antibody [EPR2762(2)]

Western blot abreview for Anti-Mib1/Mindbomb antibody [EPR2762(2)]

Dissociation constant (K_D)

Post-translational
modifications