Anti-MIF antibody (ab36146)
Key features and details
- Goat polyclonal to MIF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-MIF antibody
See all MIF primary antibodies -
Description
Goat polyclonal to MIF -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Sheep, Chicken
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Immunogen
Synthetic peptide:
NAANVGWNNSTFA
, corresponding to C terminal amino acids 102/114 of Human MIF -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
1 hour primary incubation is recommended for this product. |
| Notes |
|---|
|
WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. -
Involvement in disease
Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. -
Sequence similarities
Belongs to the MIF family. -
Cellular localization
Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. - Information by UniProt
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Database links
- Entrez Gene: 100857237 Chicken
- Entrez Gene: 4282 Human
- Entrez Gene: 17319 Mouse
- Entrez Gene: 780466 Sheep
- Omim: 153620 Human
- SwissProt: Q02960 Chicken
- SwissProt: P14174 Human
- SwissProt: P34884 Mouse
see all -
Alternative names
- GIF antibody
- GLIF antibody
- Glycosylation inhibiting factor antibody
see all
Images
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Western blot - Anti-MIF antibody (ab36146)Anti-MIF antibody (ab36146) at 0.01 µg/ml + Human thymus lysate at 35 µg
Predicted band size: 13 kDa
Observed band size: 13 kDa
Datasheets and documents
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Datasheet download
References (2)
ab36146 has been referenced in 2 publications.
- Wang Y et al. A nuclease that mediates cell death induced by DNA damage and poly(ADP-ribose) polymerase-1. Science 354:N/A (2016). PubMed: 27846469
- Greco TM et al. Quantitative mass spectrometry-based proteomics reveals the dynamic range of primary mouse astrocyte protein secretion. J Proteome Res 9:2764-74 (2010). PubMed: 20329800
