Recombinant Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)] (ab192877)
![Flow Cytometry (Intracellular) - Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)] (ab192877)](https://www.abcam.com/ps/products/192/ab192877/Images/ab192877-230669-anti-macrophage-inflammatory-protein-3-beta-antibody-epr70442-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)] (ab192877)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7044(2)] to MIP-3 beta/CCL19
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)]
See all MIP-3 beta/CCL19 primary antibodies -
Description
Rabbit monoclonal [EPR7044(2)] to MIP-3 beta/CCL19 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A549 cells; Human MIP-3 beta/CCL19 recombinant protein (aa22-98).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7044(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
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| Flow Cyt (Intra) |
1/170.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
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Flow Cyt (Intra)
1/170. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. -
Tissue specificity
Expressed at high levels in the lymph nodes, thymus and appendix. Intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6363 Human
- Omim: 602227 Human
- SwissProt: Q99731 Human
- Unigene: 50002 Human
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Alternative names
- Beta chemokine exodus 3 antibody
- Beta-chemokine exodus-3 antibody
- C C chemokine ligand 19 antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)] (ab192877)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedA549 cells labeling MIP-3 beta/CCL19 with ab192877 at 1/170 dilution (red)compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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![Western blot - Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)] (ab192877)](https://www.abcam.com/ps/products/192/ab192877/Images/ab192877-230668-anti-macrophage-inflammatory-protein-3-beta-antibody-epr70442-western-blot.jpg)
Western blot - Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)] (ab192877)Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)] (ab192877) at 1/20000 dilution + Human MIP-3 beta/CCL19 recombinant protein (aa22-98) at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDaBlocking/Dilution buffer: 5% NFDM /TBST.
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![Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)] (ab192877)](https://www.abcam.com/ps/products/192/ab192877/Images/ab192877-3-benefits-of-recombinant-antibodies.png)
Anti-MIP-3 beta/CCL19 antibody [EPR7044(2)] (ab192877)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab192877 has been referenced in 1 publication.
- Sun J et al. MiR-325-3p inhibits renal inflammation and fibrosis by targeting CCL19 in diabetic nephropathy. Clin Exp Pharmacol Physiol 47:1850-1860 (2020). PubMed: 32603491