Recombinant Anti-MiTF antibody [EPR9731] (ab140606)
![Western blot - Anti-MiTF antibody [EPR9731] (ab140606)](https://www.abcam.com/ps/products/140/ab140606/Images/ab140606-299479-anti-mitf-antibody-epr9731-western-blot.jpg "Western blot - Anti-MiTF antibody [EPR9731] (ab140606)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9731] to MiTF
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MiTF antibody [EPR9731]
See all MiTF primary antibodies -
Description
Rabbit monoclonal [EPR9731] to MiTF -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human MiTF. The exact sequence is proprietary.
Database link: O75030 -
Positive control
- WB: A-375, B16F0, mouse spleen, rat spleen and HeLa whole cell lysate (ab150035)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9731 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/200. Detects a band of approximately 52 kDa (predicted molecular weight: 58 kDa).
This antibody has weaker binding with mouse and rat than with human |
| Notes |
|---|
|
WB
1/200. Detects a band of approximately 52 kDa (predicted molecular weight: 58 kDa). This antibody has weaker binding with mouse and rat than with human |
Target
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Function
Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. -
Tissue specificity
Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. -
Involvement in disease
Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete. -
Sequence similarities
Belongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsPhosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4286 Human
- Entrez Gene: 17342 Mouse
- Entrez Gene: 25094 Rat
- Omim: 156845 Human
- SwissProt: O75030 Human
- SwissProt: Q08874 Mouse
- SwissProt: O88368 Rat
- Unigene: 166017 Human
see all -
Alternative names
- BHLHE32 antibody
- bHLHe32 antibody
- Class E basic helix-loop-helix protein 32 antibody
see all
Images
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Western blot - Anti-MiTF antibody [EPR9731] (ab140606)All lanes : Anti-MiTF antibody [EPR9731] (ab140606) at 1/200 dilution (purified)
Lane 1 : B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysates
Lane 2 : Rat spleen lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-MiTF antibody [EPR9731] (ab140606)](https://www.abcam.com/ps/products/140/ab140606/Images/ab140606-299459-anti-mitf-antibody-epr9731-western-blot.jpg)
Western blot - Anti-MiTF antibody [EPR9731] (ab140606)All lanes : Anti-MiTF antibody [EPR9731] (ab140606) at 1/1000 dilution (purified)
Lane 1 : A375 (Human malignant melanoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysates
Lane 4 : Mouse spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Anti-MiTF antibody [EPR9731] (ab140606)](https://www.abcam.com/ps/products/140/ab140606/Images/ab140606-4-benefits-of-recombinant-antibodies.png)
Anti-MiTF antibody [EPR9731] (ab140606)
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab140606 has been referenced in 7 publications.
- Qiao Z et al. Dysfunction of ATG7-dependent autophagy dysregulates the antioxidant response and contributes to oxidative stress-induced biological impairments in human epidermal melanocytes. Cell Death Discov 6:31 (2020). PubMed: 32377394
- Zhao C et al. Down-regulation of exosomal miR-200c derived from keratinocytes in vitiligo lesions suppresses melanogenesis. J Cell Mol Med 24:12164-12175 (2020). PubMed: 32918341
- Li W et al. MiTF is Associated with Chemoresistance to Cisplatin in A549 Lung Cancer Cells via Modulating Lysosomal Biogenesis and Autophagy. Cancer Manag Res 12:6563-6573 (2020). PubMed: 32801894
- Jeong H et al. Inhibitory effects on melanogenesis by thymoquinone are mediated through the ß-catenin pathway in B16F10 mouse melanoma cells. Int J Oncol 56:379-389 (2020). PubMed: 31789395
- Pan HY et al. Nutrient deprivation and lysosomal stress induce activation of TFEB in retinal pigment epithelial cells. Cell Mol Biol Lett 24:33 (2019). PubMed: 31160892
- Xu Z et al. MiR-876-5p regulates gastric cancer cell proliferation, apoptosis and migration through targeting WNT5A and MITF. Biosci Rep 39:N/A (2019). PubMed: 31171711
- Li M et al. Enhancing the Efficacy of Melanocortin 1 Receptor-Targeted Radiotherapy by Pharmacologically Upregulating the Receptor in Metastatic Melanoma. Mol Pharm 16:3904-3915 (2019). PubMed: 31318566