Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
![Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)](https://www.abcam.com/ps/products/110/ab110329/Images/ab110329-123639-anti-mitofilin-antibody-2e4ad5-mitochondrial-marker-western-blot.jpg "Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)")
Key features and details
- Mouse monoclonal [2E4AD5] to Mitofilin - Mitochondrial Marker
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker
See all Mitofilin primary antibodies -
Description
Mouse monoclonal [2E4AD5] to Mitofilin - Mitochondrial Marker -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- Human fibroblasts; Human cerebellum tissue; Isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; HL60 cells.
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General notes
Western blot advice:
Hydrophobic intrinsic membrane proteins such as core mtDNA-encoded proteins tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
ab110329 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
2E4AD5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 84 kDa.
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| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| IHC-P |
1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 84 kDa. |
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
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IHC-P
1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
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Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 10989 Human
- Entrez Gene: 76614 Mouse
- Entrez Gene: 312444 Rat
- Omim: 600378 Human
- SwissProt: Q16891 Human
- SwissProt: Q8CAQ8 Mouse
- SwissProt: Q3KR86 Rat
- Unigene: 148559 Human
see all -
Alternative names
- Cell proliferation inducing protein 52 antibody
- Cell proliferation-inducing gene 4/52 protein antibody
- Heart muscle protein antibody
see all
Images
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Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)All lanes : Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329) at 2 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
Lane 5 : HepG2 cell lysate at 20 µg
Predicted band size: 84 kDa
Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs. -
![Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)](https://www.abcam.com/ps/products/110/ab110329/Images/ab110329-123641-anti-mitofilin-antibody-2e4ad5-mitochondrial-marker-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)Immunocytochemistry analysis using ab110329 at 5µg/ml staining Mitofilin in Cultured Human fibroblasts. (Fixed and permeabilized) followed by a fluorescent goat-anti-mouse IgG. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)](https://www.abcam.com/ps/products/110/ab110329/Images/ab110329-123640-anti-mitofilin-antibody-2e4ad5-mitochondrial-marker-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)Immunohistological analysis using ab110329 at 1/100 dilution staining Mitofilin in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded). -
![Flow Cytometry - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)](https://www.abcam.com/ps/products/110/ab110329/Images/ab110329-123638-anti-mitofilin-antibody-2e4ad5-mitochondrial-marker-flow-cytometry.gif)
Flow Cytometry - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)Flow cytometric analysis using ab110329 at 1µg/ml staining Mitofilin in HL60 cells (blue). Isotype control antibody (red). -
![Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)](https://www.abcam.com/ps/products/110/ab110329/Images/ab110329-295100-anti-mitofilin-antibody-2e4ad5-mitochondrial-marker-western-blot.jpg)
Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)Image from Guarani V et al., Elife. 2016;5:e17163. Fig 5(A).; doi: 10.7554/eLife.17163. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/All lanes : Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
Lane 1 : Control neonatal mitochondria from skin fibroblasts
Lane 2 : Patient 2 (male) mitochondria from skin fibroblasts
Lane 3 : Patient 1 (female) mitochondria from skin fibroblasts
Predicted band size: 84 kDaClinical findings for patients 1 & 2 suggest human neonatal onset mitochondrial encephalopathy with recurrent bouts of liver disease.
Datasheets and documents
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SDS download
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Datasheet download
References (45)
ab110329 has been referenced in 45 publications.
- Koma R et al. Localization of myoglobin in mitochondria: implication in regulation of mitochondrial respiration in rat skeletal muscle. Physiol Rep 9:e14769 (2021). PubMed: 33650803
- Fiorentino TV et al. Pioglitazone corrects dysregulation of skeletal muscle mitochondrial proteins involved in ATP synthesis in type 2 diabetes. Metabolism 114:154416 (2021). PubMed: 33137378
- Zhu W et al. CD41-deficient exosomes from non-traumatic femoral head necrosis tissues impair osteogenic differentiation and migration of mesenchymal stem cells. Cell Death Dis 11:293 (2020). PubMed: 32341357
- Qin J et al. ER-mitochondria contacts promote mtDNA nucleoids active transportation via mitochondrial dynamic tubulation. Nat Commun 11:4471 (2020). PubMed: 32901010
- Lobo MJ et al. Phosphodiesterase 2A2 regulates mitochondria clearance through Parkin-dependent mitophagy. Commun Biol 3:596 (2020). PubMed: 33087821