Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
![Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)](https://www.abcam.com/ps/products/126/ab126575/Images/ab126575-140517-anti-mitofusin-1-antibody-11e91h12-western-blot.jpg "Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)")
Key features and details
- Mouse monoclonal [11E91H12] to Mitofusin 1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-Mitofusin 1 antibody [11E91H12]
See all Mitofusin 1 primary antibodies -
Description
Mouse monoclonal [11E91H12] to Mitofusin 1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human -
Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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Positive control
- H4IIE Rat cell lysate, NIH 3T3 Mouse cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. Near homogeneity as judged by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
11E91H12 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 5 µg/ml. Predicted molecular weight: 84 kDa.
Note that Anti-Mitofusin 1 antibody is not compatible with dilution in milk blocking buffer. |
| ICC/IF |
Use a concentration of 5 µg/ml.
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| Notes |
|---|
|
WB
Use a concentration of 5 µg/ml. Predicted molecular weight: 84 kDa. Note that Anti-Mitofusin 1 antibody is not compatible with dilution in milk blocking buffer. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks. -
Tissue specificity
Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers. -
Sequence similarities
Belongs to the mitofusin family. -
Post-translational
modificationsUbiquitinated by MARCH5. -
Cellular localization
Cytoplasm and Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 67414 Mouse
- Entrez Gene: 192647 Rat
- SwissProt: Q811U4 Mouse
- SwissProt: Q8R4Z9 Rat
- Unigene: 290414 Mouse
- Unigene: 160939 Rat
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Alternative names
- Fzo homolog antibody
- Hfzo1 antibody
- Hfzo2 antibody
see all
Images
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Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)All lanes : Anti-Mitofusin 1 antibody [11E91H12] (ab126575) at 5 µg/ml
Lane 1 : H4IIE Rat cell lysate
Lane 2 : NIH 3T3 Mouse cell lysate
Lane 3 : Mouse embryonic fibroblast cell line, wild-type
Lane 4 : Mouse embryonic fibroblast cell line, Mitofusin 1-deficient
Lane 5 : Mouse embryonic fibroblast cell line, Mitofusin 2-deficient
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat-anti-Mouse HRP at 1/3000 dilution
Predicted band size: 84 kDa
Lower panel: Anti-Actin antibody (ab3280) at 0.5µg/mL. -
![Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)](https://www.abcam.com/ps/products/126/ab126575/Images/ab126575-140516-anti-mitofusin-1-antibody-11e91h12-western-blot.jpg)
Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)All lanes : Anti-Mitofusin 1 antibody [11E91H12] (ab126575) at 5 µg/ml
Lane 1 : H4IIE Rat cell lysate
Lane 2 : Mouse embryonic fibroblast cell line, wild-type
Lane 3 : Mouse embryonic fibroblast cell line, Mitofusin 1-deficient
Lane 4 : Mouse embryonic fibroblast cell line, Mitofusin 2-deficient
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat-anti-Mouse HRP at 1/3000 dilution
Predicted band size: 84 kDa
Left panel: 4% milk was used as the blocking agent throughout the western blot. Right panel: 4% milk was used for the initial block and for the secondary antibody incubation, but ab126575 was diluted in Abcam’s Blocking Buffer (ab126587). Note that Anti-Mitofusin 1 antibody is not compatible with dilution in milk blocking buffer. -
![Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)](https://www.abcam.com/ps/products/126/ab126575/Images/ab126575-140515-anti-mitofusin-1-antibody-11e91h12-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)ab126575 at 5 µg/ml (2 hrs) staining Mitofusin 1 in paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) Mouse embryonic fibroblast cells by Immunofluorescence. Secondary: 1/2000 AlexaFluor® 488 goat anti-mouse IgG (H+L). DAPI was used to stain the cell nuclei (blue). Abcam Blocking Buffer (ab126587) was used throughout.
Protocols
Datasheets and documents
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Datasheet download
References (10)
ab126575 has been referenced in 10 publications.
- Monsalves-Alvarez M et al. ß-Hydroxybutyrate Increases Exercise Capacity Associated with Changes in Mitochondrial Function in Skeletal Muscle. Nutrients 12:N/A (2020). PubMed: 32610627
- Troiano A et al. ZSCAN4+ mouse embryonic stem cells have an oxidative and flexible metabolic profile. EMBO Rep 21:e48942 (2020). PubMed: 32424995
- Manfredi LH et al. G protein-coupled receptor kinase 2 regulates mitochondrial bioenergetics and impairs myostatin-mediated autophagy in muscle cells. Am J Physiol Cell Physiol 317:C674-C686 (2019). PubMed: 31268780
- Lu Y et al. Mitophagy is required for brown adipose tissue mitochondrial homeostasis during cold challenge. Sci Rep 8:8251 (2018). PubMed: 29844467
- Chun SK et al. Loss of sirtuin 1 and mitofusin 2 contributes to enhanced ischemia/reperfusion injury in aged livers. Aging Cell N/A:e12761 (2018). PubMed: 29774638
- Mencarelli C et al. RanBP1 Couples Nuclear Export and Golgi Regulation through LKB1 to Promote Cortical Neuron Polarity. Cell Rep 24:2529-2539.e4 (2018). PubMed: 30184488
- Chakraborty PK et al. Cystathionine ß-synthase regulates mitochondrial morphogenesis in ovarian cancer. FASEB J 32:4145-4157 (2018). PubMed: 29494264
- Yao PJ et al. Sonic hedgehog pathway activation increases mitochondrial abundance and activity in hippocampal neurons. Mol Biol Cell 28:387-395 (2017). PubMed: 27932496
- Jacobi D et al. Hepatic Bmal1 Regulates Rhythmic Mitochondrial Dynamics and Promotes Metabolic Fitness. Cell Metab 22:709-20 (2015). PubMed: 26365180
- Magnoni R et al. Late onset motoneuron disorder caused by mitochondrial Hsp60 chaperone deficiency in mice. Neurobiol Dis 54:12-23 (2013). WB ; Mouse . PubMed: 23466696
