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    products/primary-antibodies/mitofusin-2-antibody-niar164-ab124773.pdf

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RecombinantRabMAb

Recombinant Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

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  • SDS
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Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
  • Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
  • Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
  • Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [NIAR164] to Mitofusin 2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Preparation
Product image
Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer) (ab110169)
Protein
Product image
Recombinant Human Mitofusin 2 protein (ab153026)
Primary
Product image
Anti-DRP1 antibody [EPR19274] (ab184247)

View more associated products

Overview

  • Product name

    Anti-Mitofusin 2 antibody [NIAR164]
    See all Mitofusin 2 primary antibodies
  • Description

    Rabbit monoclonal [NIAR164] to Mitofusin 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Mitofusin 2. The exact sequence is proprietary.
    Database link: O95140

  • Positive control

    • WB: Mouse brain lysate, mouse kidney lysate, rat brain lysate, HeLa cell lysate, Jurkat cell lysate, HEK293 cell lysate, Raji cell lysate, rat primary neurons cell lysate. ICC/IF: HEK293 cells. IHC-P: Human kidney tissue.
  • General notes

    This Mitofusin 2 antibody (ab124773) was developed as part of a collaboration between the National Institutes of Health and the lab of Paritosh Ghosh.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    NIAR164
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitophagy fission and fusion
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Mitofusin 2 antibody [NIAR164] - BSA and Azide free (ab219730)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Mitofusin 2 protein (ab153026)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (5)
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF (1)
1/300.

For unpurified, use 1/100 - 1/250.

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF
1/300.

For unpurified, use 1/100 - 1/250.

Target

  • Function

    Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
  • Tissue specificity

    Ubiquitous; expressed at low level. Highly expressed in heart and kidney.
  • Involvement in disease

    Charcot-Marie-Tooth disease 2A2
    Neuropathy, hereditary motor and sensory, 6A
  • Sequence similarities

    Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
    Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
  • Post-translational
    modifications

    Phosphorylated by PINK1.
    Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.
  • Cellular localization

    Mitochondrion outer membrane. Colocalizes with BAX during apoptosis.
  • Target information above from: UniProt accession O95140 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9927 Human
    • Entrez Gene: 170731 Mouse
    • Entrez Gene: 64476 Rat
    • Omim: 608507 Human
    • SwissProt: O95140 Human
    • SwissProt: Q80U63 Mouse
    • SwissProt: Q8R500 Rat
    • Unigene: 376681 Human
    • Unigene: 154312 Mouse
    • Unigene: 437499 Mouse
    • Unigene: 8570 Rat
    see all
  • Alternative names

    • CMT2A antibody
    • CMT2A2 antibody
    • CPRP 1 antibody
    • CPRP1 antibody
    • EC 3.6.5.- antibody
    • Fzo antibody
    • HSG antibody
    • hyperplasia suppressor gene antibody
    • Hypertension related protein 1 antibody
    • KIAA0214 antibody
    • MARF antibody
    • MFN 2 antibody
    • Mfn2 antibody
    • MFN2_HUMAN antibody
    • Mitochondrial assembly regulatory factor antibody
    • Mitofusin-2 antibody
    • Mitofusin2 antibody
    • Transmembrane GTPase MFN2 antibody
    see all

Images

  • Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
    Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
    All lanes : Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution (purified)

    Lane 1 : mouse brain lysate
    Lane 2 : mouse kidney lysate
    Lane 3 : rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 86 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
    Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

    Immunofluorescence staining of HEK293 cells with purified ab124773 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab124773 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
    Immunohistochemical staining of paraffin embedded human kidney with purified ab124773 at a working dilution of 1/300. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
    Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
    All lanes : Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HEK293 cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 86 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
    Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)This image is courtesy of an anonymous Abreview
    Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/1000 dilution (unpurified) + Rat primary neurons cell lysate at 20 µg

    Secondary
    Anti-rabbit IgG HRP conjugate at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 86 kDa


    Exposure time: 30 seconds

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

    Unpurified ab124773, at 1/50, staining Mitofusin 2 in formalin fixed paraffin embedded Human kidney tissue using immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
    Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (51)

Publishing research using ab124773? Please let us know so that we can cite the reference in this datasheet.

ab124773 has been referenced in 51 publications.

  • Zhang XL  et al. Anti-inflammatory and neuroprotective effects of natural cordycepin in rotenone-induced PD models through inhibiting Drp1-mediated mitochondrial fission. Neurotoxicology 84:1-13 (2021). PubMed: 33549657
  • Zhou M  et al. Acupoint catgut embedding improves senescence in a rat model of ageing by regulating mitophagy via the PINK1 pathway. J Cell Mol Med 25:3816-3828 (2021). PubMed: 33645011
  • Feng L  et al. Hepatic Knockdown of Endothelin Type A Receptor (ETAR) Ameliorates Hepatic Insulin Resistance and Hyperglycemia Through Suppressing p66Shc-Mediated Mitochondrial Fragmentation in High-Fat Diet-Fed Mice. Diabetes Metab Syndr Obes 14:963-981 (2021). PubMed: 33688230
  • Frankowski H  et al. Knock-Down of HDAC2 in Human Induced Pluripotent Stem Cell Derived Neurons Improves Neuronal Mitochondrial Dynamics, Neuronal Maturation and Reduces Amyloid Beta Peptides. Int J Mol Sci 22:N/A (2021). PubMed: 33802405
  • Gordillo GM  et al. Mitochondria as Target for Tumor Management of Hemangioendothelioma. Antioxid Redox Signal 34:137-153 (2021). PubMed: 32597200
View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Mitofusin 2 antibody [NIAR164]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Cell Line)
Permeabilization
Yes - 0.3% Triton X
Specification
HeLa Cell Line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Süleyman Bozkurt

Verified customer

Submitted Feb 18 2022

Western blot abreview for Anti-Mitofusin 2 antibody [NIAR164]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - other (whole cortex)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
whole cortex
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 6% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Gilad Levy

Verified customer

Submitted Oct 07 2021

Western blot abreview for Anti-Mitofusin 2 antibody [NIAR164]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Dopaminergic Neurons)
Gel Running Conditions
Reduced Denaturing (NuPage 4-12% MES buffer)
Loading amount
30 µg
Specification
Dopaminergic Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 10 2020

Western blot abreview for Anti-Mitofusin 2 antibody [NIAR164]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293R, HCT116)
Gel Running Conditions
Reduced Denaturing
Loading amount
35 µg
Specification
HEK293R, HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 31 2019

Western blot abreview for Anti-Mitofusin 2 antibody [NIAR164]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Pig Tissue lysate - other (Mitochondria)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Mitochondria
Blocking step
Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 19 2016

Western blot abreview for Anti-Mitofusin 2 antibody [NIAR164]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary Neurons)
Loading amount
20 µg
Specification
Primary Neurons
Gel Running Conditions
Reduced Denaturing (4-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 15 2013

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