Recombinant Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
![Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)](https://www.abcam.com/ps/products/124/ab124773/Images/ab124773-254357-anti-mitofusin-2-antibody-niar164-western-blot.jpg "Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [NIAR164] to Mitofusin 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Mitofusin 2 antibody [NIAR164]
See all Mitofusin 2 primary antibodies -
Description
Rabbit monoclonal [NIAR164] to Mitofusin 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Mitofusin 2. The exact sequence is proprietary.
Database link: O95140 -
Positive control
- WB: Mouse brain lysate, mouse kidney lysate, rat brain lysate, HeLa cell lysate, Jurkat cell lysate, HEK293 cell lysate, Raji cell lysate, rat primary neurons cell lysate. ICC/IF: HEK293 cells. IHC-P: Human kidney tissue.
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General notes
This Mitofusin 2 antibody (ab124773) was developed as part of a collaboration between the National Institutes of Health and the lab of Paritosh Ghosh.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
NIAR164 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (5) |
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF | (1) |
1/300.
For unpurified, use 1/100 - 1/250. |
| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
1/300. For unpurified, use 1/100 - 1/250. |
Target
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Function
Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions. -
Tissue specificity
Ubiquitous; expressed at low level. Highly expressed in heart and kidney. -
Involvement in disease
Charcot-Marie-Tooth disease 2A2
Neuropathy, hereditary motor and sensory, 6A -
Sequence similarities
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
Contains 1 dynamin-type G (guanine nucleotide-binding) domain. -
Post-translational
modificationsPhosphorylated by PINK1.
Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion. -
Cellular localization
Mitochondrion outer membrane. Colocalizes with BAX during apoptosis. - Information by UniProt
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Database links
- Entrez Gene: 9927 Human
- Entrez Gene: 170731 Mouse
- Entrez Gene: 64476 Rat
- Omim: 608507 Human
- SwissProt: O95140 Human
- SwissProt: Q80U63 Mouse
- SwissProt: Q8R500 Rat
- Unigene: 376681 Human
see all -
Alternative names
- CMT2A antibody
- CMT2A2 antibody
- CPRP 1 antibody
see all
Images
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Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)All lanes : Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution (purified)
Lane 1 : mouse brain lysate
Lane 2 : mouse kidney lysate
Lane 3 : rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 86 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)](https://www.abcam.com/ps/products/124/ab124773/Images/ab124773-253446-anti-mitofusin-2-antibody-niar164-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)Immunofluorescence staining of HEK293 cells with purified ab124773 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab124773 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)](https://www.abcam.com/ps/products/124/ab124773/Images/ab124773-253422-anti-mitofusin-2-antibody-niar164-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)Immunohistochemical staining of paraffin embedded human kidney with purified ab124773 at a working dilution of 1/300. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)](https://www.abcam.com/ps/products/124/ab124773/Images/ab124773-254354-anti-mitofusin-2-antibody-niar164-western-blot.jpg)
Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)All lanes : Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution (purified)
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 86 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)](https://www.abcam.com/ps/products/124/ab124773/Images/ab124773-194353-anti-mitofusin-2-antibody-niar164-western-blot.jpg)
Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)This image is courtesy of an anonymous AbreviewAnti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/1000 dilution (unpurified) + Rat primary neurons cell lysate at 20 µg
Secondary
Anti-rabbit IgG HRP conjugate at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 86 kDa
Exposure time: 30 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)](https://www.abcam.com/ps/products/124/ab124773/Images/ab124773-138638-anti-mitofusin-2-antibody-niar164-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)Unpurified ab124773, at 1/50, staining Mitofusin 2 in formalin fixed paraffin embedded Human kidney tissue using immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Mitofusin 2 antibody [NIAR164] (ab124773)](https://www.abcam.com/ps/products/124/ab124773/Images/ab124773-8-benefits-of-recombinant-antibodies.png)
Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (51)
ab124773 has been referenced in 51 publications.
- Zhang XL et al. Anti-inflammatory and neuroprotective effects of natural cordycepin in rotenone-induced PD models through inhibiting Drp1-mediated mitochondrial fission. Neurotoxicology 84:1-13 (2021). PubMed: 33549657
- Zhou M et al. Acupoint catgut embedding improves senescence in a rat model of ageing by regulating mitophagy via the PINK1 pathway. J Cell Mol Med 25:3816-3828 (2021). PubMed: 33645011
- Feng L et al. Hepatic Knockdown of Endothelin Type A Receptor (ETAR) Ameliorates Hepatic Insulin Resistance and Hyperglycemia Through Suppressing p66Shc-Mediated Mitochondrial Fragmentation in High-Fat Diet-Fed Mice. Diabetes Metab Syndr Obes 14:963-981 (2021). PubMed: 33688230
- Frankowski H et al. Knock-Down of HDAC2 in Human Induced Pluripotent Stem Cell Derived Neurons Improves Neuronal Mitochondrial Dynamics, Neuronal Maturation and Reduces Amyloid Beta Peptides. Int J Mol Sci 22:N/A (2021). PubMed: 33802405
- Gordillo GM et al. Mitochondria as Target for Tumor Management of Hemangioendothelioma. Antioxid Redox Signal 34:137-153 (2021). PubMed: 32597200