Anti-MKLP1 antibody (ab9259)
Key features and details
- Rabbit polyclonal to MKLP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MKLP1 antibody
See all MKLP1 primary antibodies -
Description
Rabbit polyclonal to MKLP1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla
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Immunogen
Synthetic peptide corresponding to Human MKLP1 conjugated to keyhole limpet haemocyanin.
Database link: Q02241 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 110 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 110 kDa. |
Target
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Function
Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Localizes to the interzone of mitotic spindles. -
Sequence similarities
Belongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > spindle. - Information by UniProt
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Database links
- Entrez Gene: 9493 Human
- Omim: 605064 Human
- SwissProt: Q02241 Human
- Unigene: 270845 Human
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Alternative names
- CHO 1 antibody
- CHO1 antibody
- KIF 23 antibody
see all
Images
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Western blot - Anti-MKLP1 antibody (ab9259)All lanes : Anti-MKLP1 antibody (ab9259) at 0.4 µg/ml
Lane 1 : HeLa cell lysates treated with Nocodazole
Lane 2 : HeLa cell lysates
Lysates/proteins at 50 µg per lane.
Predicted band size: 110 kDa
Exposure time: 3 minutesDetection: Chemiluminescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab9259 has been referenced in 2 publications.
- Mollova M et al. Cardiomyocyte proliferation contributes to heart growth in young humans. Proc Natl Acad Sci U S A 110:1446-51 (2013). PubMed: 23302686
- Lordier L et al. Megakaryocyte endomitosis is a failure of late cytokinesis related to defects in the contractile ring and Rho/Rock signaling. Blood 112:3164-74 (2008). PubMed: 18684864