Recombinant Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)
![Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)](https://www.abcam.com/ps/products/240/ab240178/Images/ab240178-3-ab174304255479174304.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10879] to MKLP1 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MKLP1 antibody [EPR10879] - BSA and Azide free
See all MKLP1 primary antibodies -
Description
Rabbit monoclonal [EPR10879] to MKLP1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab240178 is the carrier-free version of ab174304.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10879 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 110 kDa.
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| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 110 kDa. |
Target
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Function
Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Localizes to the interzone of mitotic spindles. -
Sequence similarities
Belongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > spindle. - Information by UniProt
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Database links
- Entrez Gene: 9493 Human
- Entrez Gene: 71819 Mouse
- Entrez Gene: 315740 Rat
- Omim: 605064 Human
- SwissProt: Q02241 Human
- Unigene: 270845 Human
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Alternative names
- CHO 1 antibody
- CHO1 antibody
- KIF 23 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)
Immunofluorescence staining of HeLa cells with purified ab174304 at a working dilution of 1 in 400, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab174304 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174304).
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![Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)](https://www.abcam.com/ps/products/240/ab240178/Images/ab240178-2-ab174304199498ab174304b.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178) -
![Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)](https://www.abcam.com/ps/products/240/ab240178/Images/ab240178-318960-2401784444.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)Immunofluorescence staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells with purified ab174304 at a working dilution of 1 in 400, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab174304 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab174304).
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![Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)](https://www.abcam.com/ps/products/240/ab240178/Images/ab240178-4-benefits-of-recombinant-antibodies.png)
Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab240178 has not yet been referenced specifically in any publications.
