Recombinant Anti-MLD antibody [EPR9681] (ab167169)
![Western blot - Anti-MLD antibody [EPR9681] (ab167169)](https://www.abcam.com/ps/products/167/ab167169/Images/ab167169-412770-anti-mld-antibody-epr9681-western-blot-human-knockout-lysate.jpg "Western blot - Anti-MLD antibody [EPR9681] (ab167169)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9681] to MLD
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MLD antibody [EPR9681]
See all MLD primary antibodies -
Description
Rabbit monoclonal [EPR9681] to MLD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T, T47D and HepG2 whole cell lysate (ab7900). IHC-P: Human breast carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9681 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
DEGS1 is a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. It contains three His containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It has sphingolipid-delta-4-desaturase activity and converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine). -
Cellular localization
Endoplasmic reticulum and Mitochondrial -
Database links
- Entrez Gene: 8560 Human
- Omim: 615843 Human
- SwissProt: O15121 Human
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Alternative names
- Degenerative spermatocyte homolog 1 antibody
- Degenerative spermatocyte homolog 1 lipid desaturase antibody
- Degenerative spermatocyte homolog lipid desaturase antibody
see all
Images
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Western blot - Anti-MLD antibody [EPR9681] (ab167169)All lanes : Anti-MLD antibody [EPR9681] (ab167169) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : DEGS1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDaLanes 1-4: Merged signal (red and green). Green - ab167169 observed at 38 kDa. Red - loading control ab8245 observed at 36 kDa.
ab167169 Anti-MLD antibody [EPR9681] was shown to specifically react with MLD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266481 (knockout cell lysate ab257918) was used. Wild-type and MLD knockout samples were subjected to SDS-PAGE. ab167169 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] (ab167169)](https://www.abcam.com/ps/products/167/ab167169/Images/ab167169-165495-anti-degs1-antibody-epr9681-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] (ab167169)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling MLD with ab167169 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-MLD antibody [EPR9681] (ab167169)](https://www.abcam.com/ps/products/167/ab167169/Images/ab167169-165496-anti-degs1-antibody-epr9681-western-blot.jpg)
Western blot - Anti-MLD antibody [EPR9681] (ab167169)All lanes : Anti-MLD antibody [EPR9681] (ab167169) at 1/1000 dilution
Lane 1 : 293T cell lysates
Lane 2 : T47D cell lysates
Lane 3 : HepG2 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 38 kDa -
![Anti-MLD antibody [EPR9681] (ab167169)](https://www.abcam.com/ps/products/167/ab167169/Images/ab167169-4-benefits-of-recombinant-antibodies.png)
Anti-MLD antibody [EPR9681] (ab167169)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab167169 has been referenced in 5 publications.
- Hayashi Y et al. Dihydroceramide Δ4-Desaturase 1 Is Not Involved in SARS-CoV-2 Infection. Biol Pharm Bull 45:1559-1563 (2022). PubMed: 36184516
- Muñoz-Guardiola P et al. The anti-cancer drug ABTL0812 induces ER stress-mediated cytotoxic autophagy by increasing dihydroceramide levels in cancer cells. Autophagy 17:1349-1366 (2021). PubMed: 32397857
- White-Gilbertson S et al. Genetic and pharmacological inhibition of acid ceramidase prevents asymmetric cell division by neosis. J Lipid Res N/A:N/A (2019). PubMed: 30988134
- Karsai G et al. DEGS1-associated aberrant sphingolipid metabolism impairs nervous system function in humans. J Clin Invest 129:1229-1239 (2019). PubMed: 30620338
- Snider JM et al. Flux analysis reveals regulation of the sphingolipid network by doxorubicin in breast cancer cells. J Lipid Res N/A:N/A (2018). PubMed: 30573560