Recombinant Anti-MMP1 antibody [EP1249Y] (ab134184)
![Western blot - Anti-MMP1 antibody [EP1249Y] (ab134184)](https://www.abcam.com/ps/products/134/ab134184/Images/ab134184-258975-anti-mmp1-antibody-ep1249y-western-blot.jpg "Western blot - Anti-MMP1 antibody [EP1249Y] (ab134184)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1249Y] to MMP1
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MMP1 antibody [EP1249Y]
See all MMP1 primary antibodies -
Description
Rabbit monoclonal [EP1249Y] to MMP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MMP1 aa 1-100 (N terminal). The exact sequence is proprietary. The immunogen amino acid sequence is within the propeptide sequence.
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Positive control
- WB: A431 cell lysate. IP: MDA-MB-231.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1249Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 54 kDa.
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| IP |
1/20.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 54 kDa. |
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IP
1/20. |
Target
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Function
Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsUndergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4312 Human
- Omim: 120353 Human
- SwissProt: P03956 Human
- Unigene: 83169 Human
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Alternative names
- 27 kDa interstitial collagenase antibody
- CLG antibody
- CLGN antibody
see all
Images
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Western blot - Anti-MMP1 antibody [EP1249Y] (ab134184)All lanes : Anti-MMP1 antibody [EP1249Y] (ab134184) at 1/500 dilution
Lane 1 : MDA-MB-231 (human breast adenocarcinoma epithelial) whole cell lysate
Lane 2 : Lysate from HeLa cells untreated with TPA
Lane 3 : HeLa treated with 100 ng/mL TPA for 10 hours cell lysate
Lane 4 : Human colon cancer tissue lysate
Lane 5 : Human breast cancer tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 30 secondsBlocking and dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-MMP1 antibody [EP1249Y] (ab134184)](https://www.abcam.com/ps/products/134/ab134184/Images/ab134184-148508-anti-mmp1-antibody-ep1249y-western-blot.jpg)
Western blot - Anti-MMP1 antibody [EP1249Y] (ab134184)Anti-MMP1 antibody [EP1249Y] (ab134184) at 1/1000 dilution + A431 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDa -
![Immunoprecipitation - Anti-MMP1 antibody [EP1249Y] (ab134184)](https://www.abcam.com/ps/products/134/ab134184/Images/ab134184-5-anti-mmp1-antibody-ep1249y-immunoprecipitation-mdamb231-human.jpg)
Immunoprecipitation - Anti-MMP1 antibody [EP1249Y] (ab134184)MMP1 was immunoprecipitated from 0.35 mg MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg with 134184 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab134184 IP in MDA-MB-231 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab134184 in MDA-MB-231 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-MMP1 antibody [EP1249Y] (ab134184)](https://www.abcam.com/ps/products/134/ab134184/Images/ab134184-3-benefits-of-recombinant-antibodies.png)
Anti-MMP1 antibody [EP1249Y] (ab134184)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab134184 has been referenced in 7 publications.
- Liu Y et al. Long non-coding RNA HULC regulates TLR4 expression by acting as ceRNA to attract miR-663b in skin fibroblasts of pediatric burns. Am J Transl Res 13:2499-2510 (2021). PubMed: 34017408
- Yang Z et al. A Novel Mechanical-Based Injective Hydrogel for Treatment with Aromatase Inhibitors Caused Joint Inflammation via the NF-?B Pathway. ACS Omega 6:10242-10249 (2021). PubMed: 34056178
- Sun J et al. Silencing of long noncoding RNA HOXD-AS1 inhibits proliferation, cell cycle progression, migration and invasion of hepatocellular carcinoma cells through MEK/ERK pathway. J Cell Biochem 121:443-457 (2020). PubMed: 31231887
- Hu CH et al. Bone marrow concentrate-induced mesenchymal stem cell conditioned medium facilitates wound healing and prevents hypertrophic scar formation in a rabbit ear model. Stem Cell Res Ther 10:275 (2019). PubMed: 31462299
- Seidl CI et al. CRISPR-Cas9 targeting of MMP13 in human chondrocytes leads to significantly reduced levels of the metalloproteinase and enhanced type II collagen accumulation. Osteoarthritis Cartilage 27:140-147 (2019). PubMed: 30223022
- Waasdorp M et al. Protease-activated receptor-1 contributes to renal injury and interstitial fibrosis during chronic obstructive nephropathy. J Cell Mol Med 23:1268-1279 (2019). PubMed: 30485646
- Kidacki M et al. p120-Catenin Downregulation and PIK3CA Mutations Cooperate to Induce Invasion through MMP1 in HNSCC. Mol Cancer Res 15:1398-1409 (2017). PubMed: 28637905