Anti-MMP13 antibody (ab39012)
Key features and details
- Rabbit polyclonal to MMP13
- Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, Flow Cyt, Sandwich ELISA
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG
Overview
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Product name
Anti-MMP13 antibody
See all MMP13 primary antibodies -
Description
Rabbit polyclonal to MMP13 -
Host species
Rabbit -
Specificity
ab39012 recognizes the latent proenzyme, at 60 Kd, as well as the active form at 48 Kd, and intermediate activation forms. It does not cross react with the other MMP family members. ab39012 recognizes the Hinge region of MMP13. -
Tested applications
Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, Flow Cyt, Sandwich ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Dog, Human -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody has been peptide-affinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (11) |
Use at an assay dependent concentration.
|
| WB | (2) |
1/3000 - 1/6000. Predicted molecular weight: 54 kDa. 1/3000, when using colorimetric substrates such as BCIP/NBT - 1/6000, when using chemiluminescent substrates. Dilution optimised using Chromogenic detection. When used against the reduced protein identifies a band at 60 Kd. Note: The low endogenous protein levels may require concentration of samples by ultrafiltration or ammonium sulfate precipitation prior to Western blotting.
|
| IHC-Fr | (3) |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 24056368
|
| Flow Cyt |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| Sandwich ELISA |
Use a concentration of 0.5 µg/ml.
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab77949 as Capture. |
| Notes |
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IHC-P
Use at an assay dependent concentration. |
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WB
1/3000 - 1/6000. Predicted molecular weight: 54 kDa. 1/3000, when using colorimetric substrates such as BCIP/NBT - 1/6000, when using chemiluminescent substrates. Dilution optimised using Chromogenic detection. When used against the reduced protein identifies a band at 60 Kd. Note: The low endogenous protein levels may require concentration of samples by ultrafiltration or ammonium sulfate precipitation prior to Western blotting. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 24056368 |
|
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab77949 as Capture. |
Target
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Function
Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. -
Tissue specificity
Seems to be specific to breast carcinomas. -
Involvement in disease
Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 403763 Dog
- Entrez Gene: 4322 Human
- Entrez Gene: 17386 Mouse
- Entrez Gene: 171052 Rat
- Omim: 600108 Human
- SwissProt: P45452 Human
- SwissProt: P33435 Mouse
- SwissProt: P23097 Rat
see all -
Alternative names
- CLG 3 antibody
- CLG3 antibody
- Collagenase 3 antibody
see all
Images
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Western blot - Anti-MMP13 antibody (ab39012)All lanes : Anti-MMP13 antibody (ab39012) at 1/3000 dilution
Lane 1 : cell media from human chondrosarcoma (untreated).
Lane 2 : cell media from human chondrosarcoma (IL1 beta treated).
Predicted band size: 54 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
The loading amount is 15 ul/lane, and the condrosarcoma media was concentrated 40x. -
Sandwich ELISA - Anti-MMP13 antibody (ab39012)Standard curve for MMP13; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [181-15A12] to MMP13 (ab77949) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP13 - Hinge region (ab39012) at 0.5µg/ml.
Datasheets and documents
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SDS download
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Datasheet download
References (335)
ab39012 has been referenced in 335 publications.
- Zhao K et al. Acid-sensing ion channels regulate nucleus pulposus cell inflammation and pyroptosis via the NLRP3 inflammasome in intervertebral disc degeneration. Cell Prolif 54:e12941 (2021). PubMed: 33111436
- Huang HT et al. Intra-Articular Injection of (-)-Epigallocatechin 3-Gallate (EGCG) Ameliorates Cartilage Degeneration in Guinea Pigs with Spontaneous Osteoarthritis. Antioxidants (Basel) 10:N/A (2021). PubMed: 33530594
- Khurel-Ochir T et al. The immunoregulatory role of p21 in the development of the temporomandibular joint-osteoarthritis. Clin Exp Dent Res 7:313-322 (2021). PubMed: 33567474
- Shen H et al. Enhancing the potential of aged human articular chondrocytes for high-quality cartilage regeneration. FASEB J 35:e21410 (2021). PubMed: 33617078
- Nasi S et al. Xanthine Oxidoreductase Is Involved in Chondrocyte Mineralization and Expressed in Osteoarthritic Damaged Cartilage. Front Cell Dev Biol 9:612440 (2021). PubMed: 33634117