Recombinant Anti-MMP13 antibody [EP1263Y] (ab51072)
![Western blot - Anti-MMP13 antibody [EP1263Y] (ab51072)](https://www.abcam.com/ps/products/51/ab51072/Images/ab51072-333634-anti-mmp13-antibody-ep1263y-western-blot.jpg "Western blot - Anti-MMP13 antibody [EP1263Y] (ab51072)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1263Y] to MMP13
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MMP13 antibody [EP1263Y]
See all MMP13 primary antibodies -
Description
Rabbit monoclonal [EP1263Y] to MMP13 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MMP13 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa whole cell lysate (ab150035).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1263Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
For unpurified use at 1/500 |
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). For unpurified use at 1/500 |
Target
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Function
Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. -
Tissue specificity
Seems to be specific to breast carcinomas. -
Involvement in disease
Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4322 Human
- Omim: 600108 Human
- SwissProt: P45452 Human
- Unigene: 2936 Human
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Alternative names
- CLG 3 antibody
- CLG3 antibody
- Collagenase 3 antibody
see all
Images
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Western blot - Anti-MMP13 antibody [EP1263Y] (ab51072)Anti-MMP13 antibody [EP1263Y] (ab51072) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![Anti-MMP13 antibody [EP1263Y] (ab51072)](https://www.abcam.com/ps/products/51/ab51072/Images/ab51072-3-benefits-of-recombinant-antibodies.png)
Anti-MMP13 antibody [EP1263Y] (ab51072)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (37)
ab51072 has been referenced in 37 publications.
- Han SJ et al. Safflower Seed Extract Attenuates the Development of Osteoarthritis by Blocking NF-?B Signaling. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 33809253
- Wang XP et al. Quercetin suppresses apoptosis of chondrocytes induced by IL-1ß via inactivation of p38 MAPK signaling pathway. Exp Ther Med 21:468 (2021). PubMed: 33767763
- Aili D et al. Knockdown of long non-coding RNA KCNQ1OT1 suppresses the progression of osteoarthritis by mediating the miR-211-5p/TCF4 axis in vitro. Exp Ther Med 21:455 (2021). PubMed: 33747189
- Park E et al. Ameliorative Effects of Loganin on Arthritis in Chondrocytes and Destabilization of the Medial Meniscus-Induced Animal Model. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 33567513
- Park E et al. Antiosteoarthritic Effect of Morroniside in Chondrocyte Inflammation and Destabilization of Medial Meniscus-Induced Mouse Model. Int J Mol Sci 22:N/A (2021). PubMed: 33804203