Recombinant Anti-MMP3 antibody [EP1186Y] (ab52915)
![Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)](https://www.abcam.com/ps/products/52/ab52915/Images/ab52915-314000-anti-mmp3-antibody-ep1186y-western-blot.jpg "Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1186Y] to MMP3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MMP3 antibody [EP1186Y]
See all MMP3 primary antibodies -
Description
Rabbit monoclonal [EP1186Y] to MMP3 -
Host species
Rabbit -
Specificity
79% identities with MMP10 -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human MMP3 aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Raji, HEK293; mouse lung, placenta, brain, kidney liver lysate; rat brain, liver, spleen, kidney lysate; IHC-P: Rat kidney, mouse liver, human liver. ICC/IF: HT-29 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1186Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/20000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
|
| IHC-P | (3) |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
We recommend using a polymer-HRP conjugated secondary antibody. Incubate with ab52915 at 4C overnight. |
| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
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WB
1/1000 - 1/20000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). |
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IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. We recommend using a polymer-HRP conjugated secondary antibody. Incubate with ab52915 at 4C overnight. |
|
ICC/IF
1/100 - 1/250. |
Target
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Function
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4314 Human
- Entrez Gene: 17392 Mouse
- Entrez Gene: 171045 Rat
- Omim: 185250 Human
- SwissProt: P08254 Human
- SwissProt: P28862 Mouse
- SwissProt: P03957 Rat
- Unigene: 375129 Human
see all -
Alternative names
- CHDS6 antibody
- Matrix metalloproteinase 3 antibody
- Matrix metalloproteinase 3 preproprotein antibody
see all
Images
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Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte )whole cell lysate
Lane 2 : HEK293 (Human embryonic kidney epithelial cell ) whole cell lysate
Lane 3 : Mouse lung lysate
Lane 4 : Mouse placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 84 secondsBlocking and diluting buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)](https://www.abcam.com/ps/products/52/ab52915/Images/ab52915-256821-anti-mmp3-antibody-ep1186y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)Immunohistochemical staining of paraffin embedded rat kidney with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody [EP1186Y] (ab52915)](https://www.abcam.com/ps/products/52/ab52915/Images/ab52915-256838-anti-mmp3-antibody-ep1186y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MMP3 antibody [EP1186Y] (ab52915)Immunofluorescence staining of HT-29 cells with purified ab52915 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab52915 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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![Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)](https://www.abcam.com/ps/products/52/ab52915/Images/ab52915-314002-anti-mmp3-antibody-ep1186y-western-blot.jpg)
Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)
Lane 1 : Rat brain lysate
Lane 2 : Rat liver lysate
Lane 3 : Rat spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: Lane 1 & 2: 10 seconds
Lane 3: 3 minutes -
![Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)](https://www.abcam.com/ps/products/52/ab52915/Images/ab52915-314001-anti-mmp3-antibody-ep1186y-western-blot.jpg)
Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)All lanes : Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified)
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDaBlocking and diluting buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes
Lane 2 & 3: 4 seconds -
![Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)](https://www.abcam.com/ps/products/52/ab52915/Images/ab52915-314005-anti-mmp3-antibody-ep1186y-western-blot.jpg)
Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)Anti-MMP3 antibody [EP1186Y] (ab52915) at 0.37 µg/ml (purified) + Rat kidney lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and diluting buffer: 5% NFDM/TBST
-
![Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)](https://www.abcam.com/ps/products/52/ab52915/Images/ab52915-256958-anti-mmp3-antibody-ep1186y-western-blot.jpg)
Western blot - Anti-MMP3 antibody [EP1186Y] (ab52915)Anti-MMP3 antibody [EP1186Y] (ab52915) at 1/20000 dilution (purified) + MMP3 recombinant protein
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)](https://www.abcam.com/ps/products/52/ab52915/Images/ab52915-256820-anti-mmp3-antibody-ep1186y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)Immunohistochemical staining of paraffin embedded mouse liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)](https://www.abcam.com/ps/products/52/ab52915/Images/ab52915-256819-anti-mmp3-antibody-ep1186y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [EP1186Y] (ab52915)Immunohistochemical staining of paraffin embedded human liver with purified ab52915 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Anti-MMP3 antibody [EP1186Y] (ab52915)](https://www.abcam.com/ps/products/52/ab52915/Images/ab52915-13-benefits-of-recombinant-antibodies.png)
Anti-MMP3 antibody [EP1186Y] (ab52915)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (140)
ab52915 has been referenced in 140 publications.
- Pentz R et al. A new role for matrix metalloproteinase-3 in the NGF metabolic pathway: Proteolysis of mature NGF and sex-specific differences in the continuum of Alzheimer's pathology. Neurobiol Dis 148:105150 (2021). PubMed: 33130223
- Hou W et al. Excavating bioactivities of nanozyme to remodel microenvironment for protecting chondrocytes and delaying osteoarthritis. Bioact Mater 6:2439-2451 (2021). PubMed: 33553826
- Xue Y et al. Resveratrol Confers Vascular Protection by Suppressing TLR4/Syk/NLRP3 Signaling in Oxidized Low-Density Lipoprotein-Activated Platelets. Oxid Med Cell Longev 2021:8819231 (2021). PubMed: 33728029
- Cui H et al. Visfatin promotes intervertebral disc degeneration by inducing IL-6 expression through the ERK/JNK/p38 signalling pathways. Adipocyte 10:201-215 (2021). PubMed: 33853482
- Zhu H et al. Glucocorticoid counteracts cellular mechanoresponses by LINC01569-dependent glucocorticoid receptor-mediated mRNA decay. Sci Adv 7:N/A (2021). PubMed: 33627425