Recombinant Anti-MMP3 antibody [rMMP3/1730] (ab234405)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [rMMP3/1730] (ab234405)](https://www.abcam.com/ps/products/234/ab234405/Images/ab234405-336848-405b.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [rMMP3/1730] (ab234405)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [rMMP3/1730] to MMP3
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-MMP3 antibody [rMMP3/1730]
See all MMP3 primary antibodies -
Description
Mouse monoclonal [rMMP3/1730] to MMP3 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human MMP3. Recombinant fragment of human MMP3 (around aa 317-327) (exact sequence is proprietary).
Database link: P08254 -
Positive control
- IHC-P: Human placenta tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
rMMP3/1730 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at room temperature. |
| Notes |
|---|
|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 minutes at room temperature. |
Target
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Function
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4314 Human
- Omim: 185250 Human
- SwissProt: P08254 Human
- Unigene: 375129 Human
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Alternative names
- CHDS6 antibody
- Matrix metalloproteinase 3 antibody
- Matrix metalloproteinase 3 preproprotein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [rMMP3/1730] (ab234405)
Formalin-fixed, paraffin-embedded human placenta tissue stained for MMP3 using ab234405 at 1 μg/ml in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [rMMP3/1730] (ab234405)](https://www.abcam.com/ps/products/234/ab234405/Images/ab234405-336847-405a.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP3 antibody [rMMP3/1730] (ab234405)Formalin-fixed, paraffin-embedded human placenta tissue stained for MMP3 using ab234405 at 1 μg/ml in immunohistochemical analysis.
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![Anti-MMP3 antibody [rMMP3/1730] (ab234405)](https://www.abcam.com/ps/products/234/ab234405/Images/ab234405-3-benefits-of-recombinant-antibodies.png)
Anti-MMP3 antibody [rMMP3/1730] (ab234405)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab234405 has not yet been referenced specifically in any publications.
