Recombinant Anti-MMP7 antibody [EPR17888-101] (ab205525)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (ab205525)](https://www.abcam.com/ps/products/205/ab205525/Images/ab205525-250186-anti-mmp7-antibody-epr17888-101-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (ab205525)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17888-101] to MMP7
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MMP7 antibody [EPR17888-101]
See all MMP7 primary antibodies -
Description
Rabbit monoclonal [EPR17888-101] to MMP7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549 and BxPC-3 whole cell lysates; Human colon cancer, tonsil and prostate lysates. IHC-P: Human prostatic carcinoma and stomach carcinoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17888-101 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| WB |
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
|
| Notes |
|---|
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
Target
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Function
Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. -
Sequence similarities
Belongs to the peptidase M10A family. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4316 Human
- Omim: 178990 Human
- SwissProt: P09237 Human
- Unigene: 2256 Human
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Alternative names
- Matrilysin antibody
- Matrin antibody
- Matrix Metalloproteinase 7 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (ab205525)
Immunohistochemical analysis of paraffin-embedded Human stomach carcinoma tissue labeling MMP7 with ab205525 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human gastric carcinoma tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525)](https://www.abcam.com/ps/products/205/ab205525/Images/ab205525-250191-anti-mmp7-antibody-epr17888-101-western-blot.jpg)
Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525)All lanes : Anti-MMP7 antibody [EPR17888-101] (ab205525) at 1/2000 dilution
Lane 1 : A549 (Human lung carcinoma) whole cell lysate
Lane 2 : BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525)](https://www.abcam.com/ps/products/205/ab205525/Images/ab205525-250189-anti-mmp7-antibody-epr17888-101-western-blot.jpg)
Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525)All lanes : Anti-MMP7 antibody [EPR17888-101] (ab205525) at 1/1000 dilution
Lane 1 : Human colon cancer lysate
Lane 2 : Human tonsil lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525)](https://www.abcam.com/ps/products/205/ab205525/Images/ab205525-250188-anti-mmp7-antibody-epr17888-101-western-blot.jpg)
Western blot - Anti-MMP7 antibody [EPR17888-101] (ab205525)Anti-MMP7 antibody [EPR17888-101] (ab205525) at 1/1000 dilution + Human prostate lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (ab205525)](https://www.abcam.com/ps/products/205/ab205525/Images/ab205525-250187-anti-mmp7-antibody-epr17888-101-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (ab205525)Immunohistochemical analysis of paraffin-embedded Human prostatic carcinoma tissue labeling MMP7 with ab205525 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human prostatic carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (ab205525)](https://www.abcam.com/ps/products/205/ab205525/Images/ab205525-250185-anti-mmp7-antibody-epr17888-101-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR17888-101] (ab205525)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling MMP7 with ab205525 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on Human liver tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-MMP7 antibody [EPR17888-101] (ab205525)](https://www.abcam.com/ps/products/205/ab205525/Images/ab205525-8-benefits-of-recombinant-antibodies.png)
Anti-MMP7 antibody [EPR17888-101] (ab205525)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (20)
ab205525 has been referenced in 20 publications.
- Hong X et al. Biological effects of NODAL on endometrial cancer cells and its underlying mechanisms. Exp Ther Med 21:402 (2021). PubMed: 33717261
- Yuan S et al. Elevated matrix metalloproteinase 7 expression promotes the proliferation, motility and metastasis of tongue squamous cell carcinoma. BMC Cancer 20:33 (2020). PubMed: 31937294
- Niu Y et al. CCL25 promotes the migration and invasion of non-small cell lung cancer cells by regulating VEGF and MMPs in a CCR9-dependent manner. Exp Ther Med 19:3571-3580 (2020). PubMed: 32346420
- Hu XT et al. Overexpression of Progerin Results in Impaired Proliferation and Invasion of Non-Small Cell Lung Cancer Cells. Onco Targets Ther 13:2629-2642 (2020). PubMed: 32280239
- He Y et al. CircZNF609 enhances hepatocellular carcinoma cell proliferation, metastasis, and stemness by activating the Hedgehog pathway through the regulation of miR-15a-5p/15b-5p and GLI2 expressions. Cell Death Dis 11:358 (2020). PubMed: 32398664