Recombinant Anti-MMP7 antibody [EPR26105-26] (ab302893)

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling MMP7 with ab302893 at 1/500 dilution (1.068 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on Paneth cells of mouse small intestine is observed. The section was incubated with ab302893 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling MMP7 with ab302893 at 1/500 dilution (1.068 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control: no staining on mouse colon. The section was incubated with ab302893 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse small intestine (fresh) tissue labeling MMP7 with ab302893 at 1/100 dilution (5.34 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 µg/ml) (Green). Positive staining in the Paneth cells of mouse small intestine was observed. The nuclear counterstain was DAPI (Blue). The section was incubated with ab302893 for 60 mins at room temperature. The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). 

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 dilution (2 µg/ml). 

Dot blot analysis of MMP7 using ab302893 at 1:1000 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

Lane 1: Mouse MMP7 peptide

Exposure time: 180 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.