Recombinant Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (ab302894)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26105-26] to MMP7 - BSA and Azide free
- Suitable for: Dot blot, IHC-P, IHC-Fr
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free)
See all MMP7 primary antibodies -
Description
Rabbit monoclonal [EPR26105-26] to MMP7 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, IHC-P, IHC-Frmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF,IP or WB -
Species reactivity
Reacts with: Mouse
Does not react with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse: small intestine, colon tissue. IHC-Fr: Mouse small intestine (fresh). DB: Mouse MMP7 peptide
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General notes
ab302894 is a carrier free version of ab302893.
ab302893 does not react in: WB with human, mouse, and rat samples; IHC-P with human and rat tissues; ICC/IF, intracellular flow cytometry, and IP with mouse tissues.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26105-26 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab302894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Dot blot |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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Dot blot
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. -
Sequence similarities
Belongs to the peptidase M10A family. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 17393 Mouse
- SwissProt: Q10738 Mouse
- Unigene: 4825 Mouse
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Alternative names
- Matrilysin antibody
- Matrin antibody
- Matrix Metalloproteinase 7 antibody
see all
Images
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This data was developed using ab302893, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling MMP7 with ab302893 at 1/500 dilution (1.068 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on Paneth cells of mouse small intestine is observed. The section was incubated with ab302893 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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This data was developed using ab302893, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling MMP7 with ab302893 at 1/500 dilution (1.068 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control: no staining on mouse colon. The section was incubated with ab302893 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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This data was developed using ab302893, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse small intestine (fresh) tissue labeling MMP7 with ab302893 at 1/100 dilution (5.34 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml) (Green). Positive staining in the Paneth cells of mouse small intestine was observed. The nuclear counterstain was DAPI (Blue). The section was incubated with ab302893 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution (2 µg/ml).
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This data was developed using ab302893, the same antibody clone in a different buffer formulation.
Dot blot analysis of MMP7 using ab302893 at 1:1000 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.
Lane 1: Mouse MMP7 peptide
Exposure time: 180 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab302894 has not yet been referenced specifically in any publications.