Recombinant Anti-MMP8 antibody [EP1252Y] (ab81286)
![Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286)](https://www.abcam.com/ps/products/81/ab81286/Images/MMP8-Primary-antibodies-ab81286-11.jpg "Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1252Y] to MMP8
- Suitable for: WB, IHC-P, IP
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MMP8 antibody [EP1252Y]
See all MMP8 primary antibodies -
Description
Rabbit monoclonal [EP1252Y] to MMP8 -
Host species
Rabbit -
Specificity
WB positive for Human recombinant MMP8 but negative results for the following cell lines and tissue: HeLa, A431,K562 and bone marrow. -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat lung lysate. IHC-P: Human urinary bladder carcinoma and placenta tissues. IP: Rat lung lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1252Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (4) |
1/1000 - 1/5000. Predicted molecular weight: 53 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP |
1/50.
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| Notes |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 53 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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IP
1/50. |
Target
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Function
Can degrade fibrillar type I, II, and III collagens. -
Tissue specificity
Neutrophils. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules. - Information by UniProt
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Database links
- Entrez Gene: 4317 Human
- Entrez Gene: 17394 Mouse
- Entrez Gene: 63849 Rat
- Omim: 120355 Human
- SwissProt: P22894 Human
- SwissProt: O70138 Mouse
- SwissProt: O88766 Rat
- Unigene: 161839 Human
see all -
Alternative names
- CLG 1 antibody
- CLG1 antibody
- Collagenase 1 antibody
see all
Images
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Western blot - Anti-MMP8 antibody [EP1252Y] (ab81286)Anti-MMP8 antibody [EP1252Y] (ab81286) at 1/2000 dilution + Rat lung lysate at 10 µg
Secondary
HRP conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (ab81286)](https://www.abcam.com/ps/products/81/ab81286/Images/MMP8-Primary-antibodies-ab81286-13.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (ab81286)ab81286, at 1/100 dilution, staining MMP8 in human placenta by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] (ab81286)](https://www.abcam.com/ps/products/81/ab81286/Images/ab81286-4-anti-mmp8-antibody-ep1252y-immunoprecipitation-lung-rat.jpg)
Immunoprecipitation - Anti-MMP8 antibody [EP1252Y] (ab81286)Purified ab81286 at 1/50 dilution (2µg) immunoprecipitating MMP8 in Rat lung lysate.
Lane 1 (input): Rat lung lysate 10µg
Lane 2 (+): ab81286 + Rat lung lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab81286 in Rat lung lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 70 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (ab81286)](https://www.abcam.com/ps/products/81/ab81286/Images/MMP8-Primary-antibodies-ab81286-15.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody [EP1252Y] (ab81286)ab81286, at 1/100 dilution, staining MMP8 in human urinary bladder carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (35)
ab81286 has been referenced in 35 publications.
- Jiang Y et al. LncRNA HOXC-AS1 Sponges miR-99a-3p and Upregulates MMP8, Ultimately Promoting Gastric Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35884594
- Georgiadou A et al. Localised release of matrix metallopeptidase 8 in fatal cerebral malaria. Clin Transl Immunology 10:e1263 (2021). PubMed: 33968402
- Zhang J et al. Prolyl endopeptidase disruption reduces hepatic inflammation and oxidative stress in methionine-choline-deficient diet-induced steatohepatitis. Life Sci 270:119131 (2021). PubMed: 33516698
- Fu X et al. Qi-Long-Tian formula extract alleviates symptoms of acute high-altitude diseases via suppressing the inflammation responses in rat. Respir Res 22:52 (2021). PubMed: 33579290
- Tomaszewska E et al. Femoral µCT Analysis, Mechanical Testing and Immunolocalization of Bone Proteins in β-Hydroxy β-Methylbutyrate (HMB) Supplemented Spiny Mouse in a Model of Pregnancy and Lactation-Associated Osteoporosis. J Clin Med 10:N/A (2021). PubMed: 34768327