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Validated using a knockout cell line

Anti-Moesin antibody [MSN/492] (ab216033)

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Western blot - Anti-Moesin antibody [MSN/492] (ab216033)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] (ab216033)
  • Immunocytochemistry - Anti-Moesin antibody [MSN/492] (ab216033)
  • Western blot - Anti-Moesin antibody [MSN/492] (ab216033)
  • Western blot - Anti-Moesin antibody [MSN/492] (ab216033)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] (ab216033)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] (ab216033)

Key features and details

  • Mouse monoclonal [MSN/492] to Moesin
  • Suitable for: ICC, WB, IHC-P
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Moesin antibody [MSN/492]
    See all Moesin primary antibodies

  • Description

    Mouse monoclonal [MSN/492] to Moesin

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Rat

  • Immunogen

    Recombinant full length protein corresponding to Human Moesin aa 1 to the C-terminus.
    Database link: P26038

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human melanoma, Human testicular carcinoma and Human placenta tissues; ICC: HeLa cells; WB: HeLa, Jurkat and PC-3 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from bioreactor concentrate.

  • Clonality

    Monoclonal

  • Clone number

    MSN/492

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab216033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use a concentration of 1 - 2 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 68 kDa.
IHC-P
Use a concentration of 1 - 2 µg/ml.

Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
Notes
ICC
Use a concentration of 1 - 2 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 68 kDa.
IHC-P
Use a concentration of 1 - 2 µg/ml.

Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

Target

  • Function

    Probably involved in connections of major cytoskeletal structures to the plasma membrane.

  • Tissue specificity

    In all tissues and cultured cells studied.

  • Sequence similarities

    Contains 1 FERM domain.

  • Post-translational
    modifications

    Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.

  • Cellular localization

    Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
  • Information by UniProt

  • Database links

    • Alternative names

      • Epididymis luminal protein 70 antibody
      • HEL70 antibody
      • Membrane organizing extension spike protein antibody
      • Membrane-organizing extension spike protein antibody
      • MOES_HUMAN antibody
      • Moesin antibody
      • Moesin/anaplastic lymphoma kinase fusion protein antibody
      • Msn antibody
      • MSN/ALK fusion antibody
      see all

    Images

    • Western blot - Anti-Moesin antibody [MSN/492] (ab216033)
      Western blot - Anti-Moesin antibody [MSN/492] (ab216033)
      All lanes : Anti-Moesin antibody [MSN/492] (ab216033) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : MSN knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 68 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?



      False colour image of Western blot: Anti-Moesin antibody [MSN/492] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab216033 was shown to bind specifically to Moesin. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in MSN knockout cell line ab265020 (knockout cell lysate ab257542). To generate this image, wild-type and MSN knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] (ab216033)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] (ab216033)

      Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human melanoma tissue labeling Moesin with ab216033 at 1 μg/mL dilution.

    • Immunocytochemistry - Anti-Moesin antibody [MSN/492] (ab216033)
      Immunocytochemistry - Anti-Moesin antibody [MSN/492] (ab216033)

      Immunocytochemistry analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Moesin with ab216033 followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

    • Western blot - Anti-Moesin antibody [MSN/492] (ab216033)
      Western blot - Anti-Moesin antibody [MSN/492] (ab216033)
      Anti-Moesin antibody [MSN/492] (ab216033) + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

      Predicted band size: 68 kDa

    • Western blot - Anti-Moesin antibody [MSN/492] (ab216033)
      Western blot - Anti-Moesin antibody [MSN/492] (ab216033)
      Anti-Moesin antibody [MSN/492] (ab216033) + PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

      Predicted band size: 68 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] (ab216033)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] (ab216033)

      Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human testicular carcinoma tissue labeling Moesin with ab216033 at 1 μg/mL dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] (ab216033)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] (ab216033)

      Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human placenta tissue labeling Moesin with ab216033 at 1 μg/ml dilution.

    References (0)

    Publishing research using ab216033? Please let us know so that we can cite the reference in this datasheet.

    ab216033 has not yet been referenced specifically in any publications.

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