Anti-Moesin antibody [MSN/492] (ab216033)
Key features and details
- Mouse monoclonal [MSN/492] to Moesin
- Suitable for: ICC, WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Moesin antibody [MSN/492]
See all Moesin primary antibodies -
Description
Mouse monoclonal [MSN/492] to Moesin -
Host species
Mouse -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Does not react with: Rat -
Immunogen
Recombinant full length protein corresponding to Human Moesin aa 1 to the C-terminus.
Database link: P26038 -
Positive control
- IHC-P: Human melanoma, Human testicular carcinoma and Human placenta tissues; ICC: HeLa cells; WB: HeLa, Jurkat and PC-3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
MSN/492 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 1 - 2 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 68 kDa.
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IHC-P |
Use a concentration of 1 - 2 µg/ml.
Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
Notes |
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ICC
Use a concentration of 1 - 2 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 68 kDa. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
Target
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Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. -
Tissue specificity
In all tissues and cultured cells studied. -
Sequence similarities
Contains 1 FERM domain. -
Post-translational
modificationsPhosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. -
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment. - Information by UniProt
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Database links
- Entrez Gene: 4478 Human
- Omim: 309845 Human
- SwissProt: P26038 Human
- Unigene: 87752 Human
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Alternative names
- Epididymis luminal protein 70 antibody
- HEL70 antibody
- Membrane organizing extension spike protein antibody
see all
Images
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All lanes : Anti-Moesin antibody [MSN/492] (ab216033) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MSN knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Moesin antibody [MSN/492] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab216033 was shown to bind specifically to Moesin. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in MSN knockout cell line ab265020 (knockout cell lysate ab257542). To generate this image, wild-type and MSN knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] (ab216033)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human melanoma tissue labeling Moesin with ab216033 at 1 μg/mL dilution.
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Immunocytochemistry analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Moesin with ab216033 followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
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Anti-Moesin antibody [MSN/492] (ab216033) + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Predicted band size: 68 kDa -
Anti-Moesin antibody [MSN/492] (ab216033) + PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Predicted band size: 68 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] (ab216033)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human testicular carcinoma tissue labeling Moesin with ab216033 at 1 μg/mL dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Moesin antibody [MSN/492] (ab216033)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human placenta tissue labeling Moesin with ab216033 at 1 μg/ml dilution.
Datasheets and documents
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Datasheet download
References (0)
ab216033 has not yet been referenced specifically in any publications.