Recombinant Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)
![Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)](https://www.abcam.com/ps/products/307/ab307162/Images/ab307162-497271-anti-monoacylglycerol-lipasemgl-antibody-epr26936-12-western-blot-human-fat-tissue-lysate-20-g.jpg "Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26936-12] to Monoacylglycerol Lipase/MGL
- Suitable for: Flow Cyt (Intra), IP, WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12]
See all Monoacylglycerol Lipase/MGL primary antibodies -
Description
Rabbit monoclonal [EPR26936-12] to Monoacylglycerol Lipase/MGL -
Host species
Rabbit -
Specificity
Not suitable for mouse and rat for WB,ICC,ICC/IF and FC.
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Tested applications
Suitable for: Flow Cyt (Intra), IP, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: DU145, U-87 MG ICC: HT-29, U-87 MG, DU 145 IHC: Human adipose tissue, Human liver IP: DU145
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General notes
This product is manufactured by ABCAM. If you require a custom buffer formulation or conjugation please contact orders@abcam.com
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26936-12 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab307162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/50.
|
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| IP |
1/30.
|
|
| WB |
1/1000. Predicted molecular weight: 33 kDa.
|
|
| IHC-P |
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/500.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/50. |
|
IP
1/30. |
|
WB
1/1000. Predicted molecular weight: 33 kDa. |
|
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/500. |
Target
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Function
Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. -
Tissue specificity
Detected in adipose tissue, lung, liver, kidney, brain and heart. -
Pathway
Glycerolipid metabolism; triacylglycerol degradation. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family. - Information by UniProt
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Database links
- Entrez Gene: 11343 Human
- Omim: 609699 Human
- SwissProt: Q99685 Human
- Unigene: 277035 Human
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Alternative names
- EC 3.1.1.23 antibody
- HU K5 antibody
- HU-K5 antibody
see all
Images
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Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)All lanes : Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162) at 1/1000 dilution
Lane 1 : Human fat tissue lysate
Lane 2 : Human breast tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 33 kDaBlocking/Dilution Buffer: 5% NFDM/TBST
Exposure time: Lane 1: 26 seconds and Lane 2: 180 seconds
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![Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)](https://www.abcam.com/ps/products/307/ab307162/Images/ab307162-497270-anti-monoacylglycerol-lipasemgl-antibody-epr26936-12-western-blot-u-87-mg-human-glioblastoma-astrocytoma-epithelial-cell-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)Lanes 1-2 : Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : LNCaP (human prostate carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 2-3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 33 kDaBuffer/Dilution Concentration: 5% NFDM/TBST
Exposure Time: Lane 1: 10 seconds, Lane 2: 26 seconds, and Lane 3: 180 seconds
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![Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)](https://www.abcam.com/ps/products/307/ab307162/Images/ab307162-497269-anti-monoacylglycerol-lipasemgl-antibody-epr26936-12-western-blot-untreated-du145-human-prostate-carcinoma-epithelial-cell-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)All lanes : Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162) at 1/1000 dilution
Lane 1 : Untreated DU145 (human prostate carcinoma epithelial cell) whole cell lysate
Lane 2 : DU145 treated with 1 µM JZL184 for 4 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 33 kDaBuffer/Dilution Concentration: 5% NFDM/TBST
Exposure time: 3 seconds
The expression of MGL is down-regulated in response to JZL treatment (PMID: 21802006).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)](https://www.abcam.com/ps/products/307/ab307162/Images/ab307162-8-anti-monoacylglycerol-lipasemgl-antibody-epr26936-12-immunohistochemistry-human-adipose-tissue.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)Immunohistochemical analysis of paraffin-embedded human adipose tissue tissue labeling Monoacylglycerol Lipase/MGL with AB307162 at 1/5000 (0.098 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human adipose tissue. The section was incubated with ab307162 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)](https://www.abcam.com/ps/products/307/ab307162/Images/ab307162-7-anti-monoacylglycerol-lipasemgl-antibody-epr26936-12-immunohistochemistry-human-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Monoacylglycerol Lipase/MGL with AB307162 at 1/5000 (0.098 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human liver (PMID: 30034939). The section was incubated with ab307162 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Immunoprecipitation - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)](https://www.abcam.com/ps/products/307/ab307162/Images/ab307162-6-anti-monoacylglycerol-lipasemgl-antibody-epr26936-12-immunoprecipitationdu145.jpg)
Immunoprecipitation - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)Monoacylglycerol Lipase/MGL was immunoprecipitated from 0.35 mg DU145 (human prostate carcinoma epithelial cell) whole cell lysate 5 ug with AB307162 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using AB307162 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: DU145 whole cell lysate 5 ug
Lane 2: abAB307162 IP in DU145 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab307162 in DU145 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
Lane 1 : Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162) at 1/1000 dilution
Lane 1 : DU145 (human prostate carcinoma epithelial cell) whole cell lysate at 5 µg
Lane 2 : DU145 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307162 in DU145 whole cell lysate
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/5000 dilution -
![Flow Cytometry (Intracellular) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)](https://www.abcam.com/ps/products/307/ab307162/Images/ab307162-5-anti-monoacylglycerol-lipasemgl-antibody-epr26936-12-flow-cytometry-u-87-mg.jpg)
Flow Cytometry (Intracellular) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) cells labeling Monoacylglycerol Lipase/MGL with AB307162 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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![Flow Cytometry (Intracellular) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)](https://www.abcam.com/ps/products/307/ab307162/Images/ab307162-4-anti-monoacylglycerol-lipasemgl-antibody-epr26936-12-flow-cytometry-du145.jpg)
Flow Cytometry (Intracellular) - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized DU145 (human prostate carcinoma epithelial cell) cells labeling Monoacylglycerol Lipase/MGL with AB307162 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)](https://www.abcam.com/ps/products/307/ab307162/Images/ab307162-3-anti-monoacylglycerol-lipasemgl-antibody-epr26936-12-immunocytochemistry-ht-29.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma epithelial cell) cells labeling Monoacylglycerol Lipase/MGL with AB307162 at 1/500 dilution (0.976 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).
Confocal image showing cytoplasmic staining in HT-29 cells line.
Low expression: HCT 116 (PMID: 22349814).
The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).
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![Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)](https://www.abcam.com/ps/products/307/ab307162/Images/ab307162-2-anti-monoacylglycerol-lipasemgl-antibody-epr26936-12-immunocytochemistry-u-87-mg.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (human glioblastoma-astrocytoma epithelial cell) cells labeling Monoacylglycerol Lipase/MGL with AB307162 at 1/500 dilution (0.976 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).
Confocal image showing cytoplasmic staining in U-87 MG line.
The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).
-
![Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)](https://www.abcam.com/ps/products/307/ab307162/Images/ab307162-1-anti-monoacylglycerol-lipasemgl-antibody-epr26936-12-immunocytochemistry-du-145.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized DU 145 (human prostate carcinoma epithelial cell) cells labeling Monoacylglycerol Lipase/MGL with AB307162 at 1/500 dilution (0.976 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).
Confocal image showing cytoplasmic staining in DU 145 cell line.
The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).
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![Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)](https://www.abcam.com/ps/products/307/ab307162/Images/ab307162-9-BenefitsofRecombinantantibodies.jpg)
Anti-Monoacylglycerol Lipase/MGL antibody [EPR26936-12] (ab307162)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab307162 has not yet been referenced specifically in any publications.