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Mouse IgG1 [CT6] - Isotype control (ab81216)

Q&A (3)References (5)

Overview

  • Product name

    Mouse IgG1 [CT6] - Isotype control
    See all Mouse isotype controls

  • Specificity

    The antibody reacts with a Guinea pig lymphocyte subset probably analog to human CD8 (cytotoxic/suppressor) subset. CD8 comprises 2 subunits, alpha and beta and exists as either an alpha/alpha homodimer or an alpha/beta heterodimer. Sequence suggests that guinea pig CD8 is more closely related to human than rat or mouse CD8.

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details

  • Immunogen

    This information is considered to be commercially sensitive.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.3
    Preservative: 0.1% Sodium azide
  • Concentration information loading...

  • Purification notes

    Membrane filtration

  • Clonality

    Monoclonal

  • Clone number

    CT6

  • Isotype

    IgG1

  • Research areas

  • Alternative names

    • Mouse Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab81216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
IHC-P
1/20.
Notes
WB
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
IHC-P
1/20.

References (5)

Publishing research using ab81216? Please let us know so that we can cite the reference in this datasheet.

ab81216 has been referenced in 5 publications.

  • Schehr JL  et al. High Specificity in Circulating Tumor Cell Identification Is Required for Accurate Evaluation of Programmed Death-Ligand 1. PLoS One 11:e0159397 (2016). PubMed: 27459545
  • Moradi F  et al. Dual mechanisms of action of the RNA-binding protein human antigen R explains its regulatory effect on melanoma cell migration. Transl Res 172:45-60 (2016). PubMed: 26970271
  • Xiao T  et al. CTCF Recruits Centromeric Protein CENP-E to the Pericentromeric/Centromeric Regions of Chromosomes through Unusual CTCF-Binding Sites. Cell Rep 12:1704-14 (2015). PubMed: 26321640
  • Scheper RJ  et al. Monoclonal antibody JSB-1 detects a highly conserved epitope on the P-glycoprotein associated with multi-drug-resistance. Int J Cancer 42:389-94 (1988). PubMed: 2458323
  • Tan BT  et al. Production of monoclonal antibodies defining guinea pig T-cell surface markers and a strain 13 Ia-like antigen: the value of immunohistological screening. Hybridoma 4:115-24 (1985). Guinea pig . PubMed: 3891587

Customer reviews and Q&As

Q&A
Submit a question

1-3 of 3 Q&A

Question

I purchased Anti-Negative control for mouse IgG1 Ab (ab81216) to assess the effect of other blocking ab on tumor cell growth.
But, what I found was cells treated with IgG1 ab also showed retarded growth similar with blocking ab.
I am wondering if this item is not suitable for functional assay such as cell proliferation due to, for example, sodium azide or something ?

Look forward to hearing from you, soon.

Read More
Answer

Thank you for contacting us.

We have not heard other reports of this, but it is possible the azide in the antibody, which is present at 0.1% in the stock solution, does have an effect on the tumor growth. Confirming this would require an experiment, for instance comparing the effect of medium containing the same amount of azide to the effect of the antibody control.

Alternatively, you could remove the azide by exchanging the buffer of the stock solution. The product ab102776 will accomplish this. Here is a URL for the product:

Click here (or use the following: https://www.abcam.com/Antibody-Concentration-Kit-1-column-ab102776.html).

Please let me know if you have other questions or concerns.

Read More

Question

Please send us CofC for these two items.

Read More
Answer

Thank you for your reply.

I have attached the certificate of compliance for ab290 and ab81216, with the lot numbers specified.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Question

Please send me CofA for the followings:

Cat# AB290 Lot# GR7657-1

Cat# AB81216 Lot# GR98155-1

Thanks

Read More
Answer

Thank you for your enquiry.

Some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Abpromise to be of high quality. Our Abpromise assures you that if our products do not perform as our datasheets say they should we offer a replacement or refund within 6 monthsof purchase. Some of our collaborators are not certified ISO9001 and so we are not able to provide a certificate of analysis for these particular products.

As the identity of our collaborators is confidential, we provide a certificate of compliance for all of our products available in our catalogue. This document certifies that our products meet the required specifications for a particular lot/batch of product.

The Customer Services and Scientific Support Teams would be very pleased to send a CoC on demand. Could you please let me know if you would stilllike to receive a CoC for these two items?

Please do not hesitate to contact me again if you have further questions.

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