Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
![Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)](https://www.abcam.com/ps/products/170/ab170190/Images/ab170190-171551-mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-flow-cytometry.jpg "Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)")
Overview
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Product name
Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control
See all Mouse isotype controls -
Specificity
This Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments. -
Tested applications
Suitable for: Flow Cyt, ELISA, IHC-P, ICC/IFmore details -
Immunogen
Recombinant full length protein. ab170190 binds specifically to DNA-binding domain of the yeast GAL4 protein transcription activator.
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General notes
This antibody clone is manufactured by Abcam.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The control performed appropriately in all materials and platforms that were tested.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Monoclonal -
Clone number
15-6E10A7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
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Alternative names
- Mouse Isotype Control
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 µg/ml.
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| ELISA |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. PubMed: 26627833
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| Notes |
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Flow Cyt
Use a concentration of 1 µg/ml. |
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ELISA
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. PubMed: 26627833 |
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ICC/IF
Use a concentration of 1 µg/ml. |
Images
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Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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![Immunocytochemistry/ Immunofluorescence - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)](https://www.abcam.com/ps/products/170/ab170190/Images/ab170190-171550-mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)Immunocytochemistry (ICC) experiments with NIH-3T3 (mouse sarcoma), COS7 (monkey kidney fibroblast), H9C2 (rat myoblast) and Hela (human adenocarcinoma) were performed with the IgG1 Isotype Control Antibody (top), no primary antibody negative control (middle), and ab14705 as a positive control (bottom).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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![ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)](https://www.abcam.com/ps/products/170/ab170190/Images/ab170190-169713-mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-elisa.jpg)
ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)Fluorescence ELISA.
An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab170190 has been referenced in 17 publications.
- Livanos AE et al. Intestinal Host Response to SARS-CoV-2 Infection and COVID-19 Outcomes in Patients With Gastrointestinal Symptoms. Gastroenterology 160:2435-2450.e34 (2021). PubMed: 33676971
- Wang Z et al. Methylprednisolone alleviates multiple sclerosis by expanding myeloid-derived suppressor cells via glucocorticoid receptor ß and S100A8/9 up-regulation. J Cell Mol Med 24:13703-13714 (2020). PubMed: 33094923
- Martens R et al. Efficient homing of T cells via afferent lymphatics requires mechanical arrest and integrin-supported chemokine guidance. Nat Commun 11:1114 (2020). PubMed: 32111837
- Sotoudeh M et al. ANTXR1 (TEM8) overexpression in gastric adenocarcinoma makes the protein a potential target of immunotherapy. Cancer Immunol Immunother 68:1597-1603 (2019). PubMed: 31520110
- D'Antonio-Chronowska A et al. In vitro Differentiation of Human iPSC-derived Retinal Pigment Epithelium Cells (iPSC-RPE). Bio Protoc 9:e3469 (2019). PubMed: 33654959