Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free (ab221848)
![Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free (ab221848)](https://www.abcam.com/ps/products/221/ab221848/Images/ab221848-282742-mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-flow-cytometry.jpg "Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free (ab221848)")
Overview
-
Product name
Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free
See all Coiled-coil domain-containing protein 113 isotype controls -
Specificity
This Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments. -
Tested applications
Suitable for: IHC-P, ICC, Flow Cytmore details -
Immunogen
Recombinant full length protein. ab221848 binds specifically to DNA-binding domain of the yeast GAL4 protein transcription activator.
-
General notes
This antibody clone is manufactured by Abcam.
ab221848 is a PBS only buffer formulated version of ab170190.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Monoclonal -
Clone number
15-6E10A7 -
Isotype
IgG1 -
Light chain type
kappa -
Alternative names
- CCDC113
- Coiled-coil domain-containing protein 113 Gene names
- DKFZp434N1418
- HSPC065
Associated products
-
Alternative Versions
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. PubMed: 26627833
|
|
| ICC |
Use a concentration of 1 µg/ml.
|
|
| Flow Cyt |
Use a concentration of 1 µg/ml.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. PubMed: 26627833 |
|
ICC
Use a concentration of 1 µg/ml. |
|
Flow Cyt
Use a concentration of 1 µg/ml. |
Images
-
Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free (ab221848)
This flow cytometry data was generated using the same antibody clone under a different buffer format (cat# ab170190).
Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
Protocols
Datasheets and documents
-
Datasheet download
References (4)
ab221848 has been referenced in 4 publications.
- Cui Y et al. The miR-20-Rest-Wnt signaling axis regulates neural progenitor cell differentiation. Sci Rep 6:23300 (2016). Flow Cyt . PubMed: 26996236
- Landa-Solís C et al. Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications. Cell Tissue Bank N/A:N/A (2015). PubMed: 26220398
- Ebisawa M et al. Significance of caveolin-1 and matrix metalloproteinase 14 gene expression in canine mammary tumours. Vet J N/A:N/A (2015). IF . PubMed: 26364240
- Douglas DN et al. Oxidative stress attenuates lipid synthesis and increases mitochondrial fatty acid oxidation in hepatoma cells infected with hepatitis C virus. J Biol Chem N/A:N/A (2015). IHC-P . PubMed: 26627833
