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  1. Link

    products/primary-antibodies/mouse-igg1-kappa-monoclonal-mg1-45-isotype-control-ab18448.pdf

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Isotype/Loading Controls Isotype Controls Mouse
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Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control (ab18448)

  • Datasheet
Q&A (5)References (6)

Product price, shipping and contact information

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Overview

  • Product name

    Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control
    See all Mouse isotype controls
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, Flow Cyt, WB, IP, ELISAmore details
  • Immunogen

    Chemical/ Small Molecule (Trinitrophenol) conjugated to KLH.

  • General notes


    The ab18448, MG1-45 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    The immunoglobulin was purified by affinity chromatography.
  • Isotype control notes

    The ab18448, MG1-45 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • Clonality

    Monoclonal
  • Clone number

    MG1-45
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Isotype/Loading Controls
    • Isotype Controls
    • Mouse
  • Alternative names

    • Mouse Isotype Control

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration. PubMed: 18006877
Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration. PubMed: 18006877

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (6)

Publishing research using ab18448? Please let us know so that we can cite the reference in this datasheet.

ab18448 has been referenced in 6 publications.

  • Silva MA  et al. Biomarker recommendation for PD-1/PD-L1 immunotherapy development in pediatric cancer based on digital image analysis of PD-L1 and immune cells. J Pathol Clin Res 6:124-137 (2020). PubMed: 31922656
  • Wang Q  et al. The mTOR Signal Pathway Is Overactivated in Human Lymphatic Malformations. Lymphat Res Biol N/A:N/A (2019). PubMed: 31381473
  • Yuan SM  et al. Over-expression of PPAR-?2 gene enhances the adipogenic differentiation of hemangioma-derived mesenchymal stem cellsin vitroandin vivo. Oncotarget 8:115817-115828 (2017). PubMed: 29383203
  • Feng H  et al. NLRX1 promotes immediate IRF1-directed antiviral responses by limiting dsRNA-activated translational inhibition mediated by PKR. Nat Immunol 18:1299-1309 (2017). PubMed: 28967880
  • Keil R  et al. Targeting of p0071 to the midbody depends on KIF3. J Cell Sci 122:1174-1183 (2009). PubMed: 19339549
  • de Jong MA  et al. Dendritic cells mediate herpes simplex virus infection and transmission through the C-type lectin DC-SIGN. J Gen Virol 89:2398-409 (2008). PubMed: 18796707

Customer reviews and Q&As

Q&A
Submit a question

1-5 of 5 Q&A

Question

Bonjour,



Voici le document rempli avec des résultats de FACS, les photos IF et le WB.

L’anticorps abcam marche en IF et WB, par contre l’isotype reconnait une bande de la même taille que K10 en WB…

Je n’ai pas testé l’isotype en IF, mais je pourrais éventuellement tester les deux AC en WB sur HEK pour voir si j’ai le même résultat qu’en FACS.



Dans l’attente d’une réponse de votre part

Read More

Abcam community

Verified customer

Asked on Sep 13 2012

Answer

Mercipour votre réponse ainsi que pour le questionnaire rempli et détaillé.

Il m'est toujours impossible de trouver une explication définitive à votre problème.
Est-ce que l'anticorps anti-K10 est également un monoclonal de souris IgG1 ?
Est-ce que ce même anticorps marque les controles négatifs (HEK) en cytométrie?
Quel controle isotype utilisez-vous avec cet anticorps?

J'espère pouvoir trouver rapidement une explication et une résolution à votre problème. N'hésitez pas à me communiquer votre opinion sur la question.

Read More

Abcam Scientific Support

Answered on Sep 13 2012

Question

Problèmes en FACS et IFavec ab9025 (primaire) et ab18448 (controle isotype).
Anticorps couplés avec kit invitrogen "zenon"

controles négatifs : HEK

marquage positif obtenus dans tous les cas de figures.

Read More

Abcam community

Verified customer

Asked on Sep 12 2012

Answer

En plus de vos résultats obtenus en Western Blot, vous serait-il possible de remplir et nous retourner le questionnaire ci-joint?

Les informations sur le protocole utilisé nous sera utile pour tenter de comprendre le problème rencontré avec les 2 anticorps ab9025 et ab18448.

Merci.

Read More

Abcam Scientific Support

Answered on Sep 12 2012

Question

Problèmes en FACS et IFavec ab9025 (primaire) et ab18448 (controle isotype).
Anticorps couplés
controles négatifs : HEK

marquage positif obtenus dans tous les cas de figures.

Read More

Abcam community

Verified customer

Asked on Sep 11 2012

Answer

Merci de nous avoir contactés.

Je vous envoie comme convenu un email auquel vous pourrez répondre et joindre vos résultats en Western Blot avec les anticorps ab9025 et ab18448.
Votre réponse me sera alors automatiquement attribuée.

Read More

Abcam Scientific Support

Answered on Sep 11 2012

Question

Phonec all: Customer asked the concentration of mouse IgG1 isotype control.

Read More

Abcam community

Verified customer

Asked on Sep 21 2011

Answer

Thank you for contacting us. Could you please specify which product you would be interested in buying? I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Sep 21 2011

Question

Phonec all: Customer asked the concentration of mouse IgG1 isotype control.

Read More

Abcam community

Verified customer

Asked on Sep 20 2011

Answer

Thank you for contacting us. I can confirm that product ab91353 can also be used as normal isotype control with human cells or in applications as stated on the datasheet. I have also sent an email to my colleague in lab about your requirements and now waiting a reply from them. I will get back to you soon. Should you have any other question please do not hesitate to contact me.

Read More

Abcam Scientific Support

Answered on Sep 20 2011

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