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Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

Q&A (2)References (31)

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Overview

  • Product name

    Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control
    See all Mouse isotype controls

  • Specificity

    MOPC-21 immunoglobulin has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live and fixed rat and human tissues.

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, Flow Cyt, IP, WBmore details

  • Immunogen

    Other Immunogen Type corresponding to Mouse. There is no immunogen for this product because it was not generated through a hybridoma. It was generated by the myeloma of a mouse that was fed mineral oil.

  • General notes

    ab18443 MOPC-21 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Isotype control notes

    ab18433 MOPC-21 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

  • Clonality

    Monoclonal

  • Clone number

    MOPC-21

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

  • Alternative names

    • Mouse Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

References (31)

Publishing research using ab18443? Please let us know so that we can cite the reference in this datasheet.

ab18443 has been referenced in 31 publications.

  • Cotton S  et al. Target Score-A Proteomics Data Selection Tool Applied to Esophageal Cancer Identifies GLUT1-Sialyl Tn Glycoforms as Biomarkers of Cancer Aggressiveness. Int J Mol Sci 22:N/A (2021). PubMed: 33562270
  • Pasquardini L  et al. A Surface Plasmon Resonance Plastic Optical Fiber Biosensor for the Detection of Pancreatic Amylase in Surgically-Placed Drain Effluent. Sensors (Basel) 21:N/A (2021). PubMed: 34063347
  • Niedermair T  et al. Impact of the Sensory and Sympathetic Nervous System on Fracture Healing in Ovariectomized Mice. Int J Mol Sci 21:N/A (2020). PubMed: 31936403
  • Guttà C  et al. Low expression of pro-apoptotic proteins Bax, Bak and Smac indicates prolonged progression-free survival in chemotherapy-treated metastatic melanoma. Cell Death Dis 11:124 (2020). PubMed: 32054850
  • Nagao M  et al. Potential Protection Against Type 2 Diabetes in Obesity Through Lower CD36 Expression and Improved Exocytosis in ß-Cells. Diabetes 69:1193-1205 (2020). PubMed: 32198214
View all Publications for this product

Customer reviews and Q&As

Q&A
Submit a question

1-2 of 2 Q&A

Question

We purchased your IgG 1 Kappa control antibody (ab18443) about 4 months ago. I was wondering if you could tell me what the lot number would be for that timeframe.

We accidentally tossed the vial and I need it for a report I'm filling out. Thanks for your help!

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Answer

Thank you for contacting us.

If you provide me with the institution that placed the order, I will be able to tell you the exact lot number you purchased. However, roughly 4 months ago we were selling lot GR54839-4.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Need IgG isotype control for IHC on paraffin sections. I have 3 mouse monoclonal antibodies which I require isotype controls for - 2 mouse are type IgG1, one is IgG1,kappa. Do I need to match for the kappa as well or can I just use a IgG1? Please can you tell me whether I can just use IgG1 isotype control for all antibodies or does the IgG1, kappa require one of the other isotype controls, such as ab18443 or ab18447?

Read More
Answer

Thank you for your enquiry. According to my literature 99% of mouse IgG immunoglobulin molecules have a kappa light chain. This is reflected in the number of IgG, kappa isotype controls in our catalogue. Therefore given that your three mouse antibodies are IgG1; with the light chain unspecified in two of them and one specified as kappa I think that it is safe to assume that you can use a single isotype control relating for all three antibodies relating to heavy chain IgG1 and kappa light chain. For example; ab18443 Mouse IgG1, kappa monoclonal (MOPC-21) - isotype control or ab18447 Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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