Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
Overview
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Product name
Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control
See all Mouse isotype controls -
Specificity
ab18415 MG2a-53 immunoglobulin reacts with KLH. The MG2a-53 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues -
Tested applications
Suitable for: IHC-P, IHC-Fr, Flow Cyt, IP, WBmore details -
Immunogen
Trinitrophenol + KLH
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General notes
ab18415 MG2a-53 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Isotype control notes
ab18415 MG2a-53 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. -
Clonality
Monoclonal -
Clone number
MG2a-53 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
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Alternative names
- Mouse Isotype Control
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
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| IHC-Fr |
Use at an assay dependent dilution.
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| Flow Cyt |
Use at an assay dependent dilution.
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| IP |
Use at an assay dependent dilution.
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| WB |
Use at an assay dependent dilution.
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| Notes |
|---|
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IHC-P
Use at an assay dependent dilution. |
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IHC-Fr
Use at an assay dependent dilution. |
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Flow Cyt
Use at an assay dependent dilution. |
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IP
Use at an assay dependent dilution. |
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WB
Use at an assay dependent dilution. |
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab18415 has been referenced in 14 publications.
- Kilinc M et al. Endogenous Syngap1 alpha splice forms promote cognitive function and seizure protection. Elife 11:N/A (2022). PubMed: 35394425
- Tossetta G et al. Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development. Cell Tissue Res 390:113-129 (2022). PubMed: 35794391
- Tossetta G et al. Ciliary Neurotrophic Factor Modulates Multiple Downstream Signaling Pathways in Prostate Cancer Inhibiting Cell Invasiveness. Cancers (Basel) 14:N/A (2022). PubMed: 36497399
- Eylert G et al. Skin regeneration is accelerated by a lower dose of multipotent mesenchymal stromal/stem cells-a paradigm change. Stem Cell Res Ther 12:82 (2021). PubMed: 33494813
- Iacobini C et al. Normalizing HIF-1α Signaling Improves Cellular Glucose Metabolism and Blocks the Pathological Pathways of Hyperglycemic Damage. Biomedicines 9:N/A (2021). PubMed: 34572324