Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)
Related conjugates and formulations
Overview
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Product name
Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade
See all Mouse isotype controls -
Specificity
This Balb/c myeloma derived clone has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues. -
Tested applications
Suitable for: IP, WB, IHC-Fr, IHC-P, Flow Cyt, ChIP/Chip, ChIPmore details -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MOPC-173 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
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Alternative names
- Mouse Isotype Control
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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ChIP/Chip |
Use at an assay dependent concentration. PubMed: 18636108
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ChIP |
Use at an assay dependent concentration. PubMed: 18636108
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Notes |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
ChIP/Chip
Use at an assay dependent concentration. PubMed: 18636108 |
ChIP
Use at an assay dependent concentration. PubMed: 18636108 |
Images
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ChIP - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)Image from Niemantsverdriet M et al., PLoS One. 2012;7(12):e50815. Fig 5.; doi: 10.1371/journal.pone.0050815. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
ChIP of ΔNp73 binding with SBE. The relative amount of ΔNp73 associated DNA as pulled down with an antibody directed against HA, is represented as a fold enrichment compared to pull-down with IgG (background, ab18413). Gene enrichment was quantified by qPCR using primers specific for the promoter regions of A) PAI-1, B) Col1a1 and C) p21WAF within the SBEs. Primers specific for PTEN (D) were used as a positive control. Pulldown antibody is shown on the x-axis, with y-axis showing fold enrichment ± SEM. * represents p-value>0.05.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (61)
ab18413 has been referenced in 61 publications.
- Bliss CM et al. A single-shot adenoviral vaccine provides hemagglutinin stalk-mediated protection against heterosubtypic influenza challenge in mice. Mol Ther 30:2024-2047 (2022). PubMed: 34999208
- Li J et al. Regulation of integrin and extracellular matrix genes by HNRNPL is necessary for epidermal renewal. PLoS Biol 19:e3001378 (2021). PubMed: 34543262
- Jayabal P et al. EZH2 suppresses endogenous retroviruses and an interferon response in cancers. Genes Cancer 12:96-105 (2021). PubMed: 34966479
- Li J et al. Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase. Nat Commun 12:896 (2021). PubMed: 33563994
- Li J et al. SPT6 promotes epidermal differentiation and blockade of an intestinal-like phenotype through control of transcriptional elongation. Nat Commun 12:784 (2021). PubMed: 33542242