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Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)

Q&A (3)References (61)
ChIP - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 488 Alexa Fluor® 647 APC Biotin FITC PerCP

    Overview

    • Product name

      Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade
      See all Mouse isotype controls

    • Specificity

      This Balb/c myeloma derived clone has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.

    • Tested applications

      Suitable for: IP, WB, IHC-Fr, IHC-P, Flow Cyt, ChIP/Chip, ChIPmore details

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab18413 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.
    ChIP/Chip
    Use at an assay dependent concentration. PubMed: 18636108
    ChIP
    Use at an assay dependent concentration. PubMed: 18636108
    Notes
    IP
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.
    ChIP/Chip
    Use at an assay dependent concentration. PubMed: 18636108
    ChIP
    Use at an assay dependent concentration. PubMed: 18636108

    Images

    • ChIP - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)
      ChIP - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)Image from Niemantsverdriet M et al., PLoS One. 2012;7(12):e50815. Fig 5.; doi: 10.1371/journal.pone.0050815. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      ChIP of ΔNp73 binding with SBE. The relative amount of ΔNp73 associated DNA as pulled down with an antibody directed against HA, is represented as a fold enrichment compared to pull-down with IgG (background, ab18413). Gene enrichment was quantified by qPCR using primers specific for the promoter regions of A) PAI-1, B) Col1a1 and C) p21WAF within the SBEs. Primers specific for PTEN (D) were used as a positive control. Pulldown antibody is shown on the x-axis, with y-axis showing fold enrichment ± SEM. * represents p-value>0.05.

    References (61)

    Publishing research using ab18413? Please let us know so that we can cite the reference in this datasheet.

    ab18413 has been referenced in 61 publications.

    • Bliss CM  et al. A single-shot adenoviral vaccine provides hemagglutinin stalk-mediated protection against heterosubtypic influenza challenge in mice. Mol Ther 30:2024-2047 (2022). PubMed: 34999208
    • Li J  et al. Regulation of integrin and extracellular matrix genes by HNRNPL is necessary for epidermal renewal. PLoS Biol 19:e3001378 (2021). PubMed: 34543262
    • Jayabal P  et al. EZH2 suppresses endogenous retroviruses and an interferon response in cancers. Genes Cancer 12:96-105 (2021). PubMed: 34966479
    • Li J  et al. Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase. Nat Commun 12:896 (2021). PubMed: 33563994
    • Li J  et al. SPT6 promotes epidermal differentiation and blockade of an intestinal-like phenotype through control of transcriptional elongation. Nat Commun 12:784 (2021). PubMed: 33542242
    View all Publications for this product

    Customer reviews and Q&As

    Q&A
    Submit a question

    1-3 of 3 Q&A

    Question

    What is the difference between ab 18413 and ab 18449?

    Read More
    Answer

    Thank you for your reply.

    They are the same antibody (same clone), just that ab18449 has been screened for low endotoxin levels for use with cell culture.

    I hope this information helps. Please contact us with any other questions.

    Read More

    Question

    What are differences between these two products that may affect a selection of an antibody control for ab 9324 for application in cultured human hepatocytes?

    Read More
    Answer

    Thank you for contacting us.

    The anti-IL6 antibody ab9324 is a mouse monoclonal IgG2a. Therefore, you will need to use a mouse monoclonal IgG2a isotype control.

    Therefore, you should use the mouse control IgG2a ab18413 and NOT the rabbit IgG ab37415.

    To learn more about isotype controls, please see the link below:

    https://www.abcam.com/index.html?pageconfig=technicalfaqs#5

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Inquiry: It is indicated in the description of ab18413 that it is Mouse Control IgG, but when you scroll down to the isotype paragraph of this product, you will see that it is IgG2a. Please clarify if ab18413 is Mouse Control IgG or Mouse Control IgG2a. The customer works with 2 primary antibodies. One is IgG2a and the second is IgG1 and she is looking for one isotype control for IHC-P that will fit both primary antibodies, that's why it is important for her to be sure that ab18413 is Mouse Control IgG and not only Mouse Control IgG2a. Thank you very much for your assistance and reply. Have a nice day.

    Read More
    Answer

    Thank you for contacting us.

    I can confirm that ab18413 is isotype IgG2a, therefore is only suitable for one of the primary antibodies the customer is using. I have asked our products team to update the datasheet.

    Apologies for any confusion.

    Read More

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