Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
![Immunocytochemistry/ Immunofluorescence - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)](https://www.abcam.com/ps/products/170/ab170192/Images/ab170192-169997-mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-immunocytochemistry-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)")
Overview
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Product name
Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control -
Specificity
ab170192, mouse IgG2b Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments. -
Tested applications
Suitable for: Flow Cyt, ICC/IF, ELISAmore details -
General notes
This antibody clone is manufactured by Abcam.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The control performed appropriately in all materials and platforms that were tested.
This product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Monoclonal -
Clone number
7E10G10 -
Isotype
IgG2b -
Light chain type
kappa
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 µg/ml.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
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Flow Cyt
Use a concentration of 1 µg/ml. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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ELISA
Use at an assay dependent concentration. |
Images
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Immunocytochemistry/ Immunofluorescence - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
Immunofluorscent analysis of NIH 3T3, COS7, H9C2 and HeLa cells using ab170192 at 1µg/ml (top); no primary antibody negative control (middle), and ab14705 as a positive control (bottom). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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![Flow Cytometry - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)](https://www.abcam.com/ps/products/170/ab170192/Images/ab170192-171552-mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-flow-cytometry.jpg)
Flow Cytometry - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)Flow cytometry experiments with 4% PFA fixed Hela (human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast)were performed with the IgG2b Isotype Control Antibody (red) and no primary antibody negative control (black). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody.
The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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![ELISA - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)](https://www.abcam.com/ps/products/170/ab170192/Images/ab170192-170000-mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-elisa.jpg)
ELISA - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mLab170192 and detecting with Alexa Fluor® conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab170192 has been referenced in 3 publications.
- Liu B et al. Sodium iodate induces ferroptosis in human retinal pigment epithelium ARPE-19 cells. Cell Death Dis 12:230 (2021). PubMed: 33658488
- Andrews JM et al. Loss of synergistic transcriptional feedback loops drives diverse B-cell cancers. EBioMedicine 71:103559 (2021). PubMed: 34461601
- Strieder-Barboza C et al. Advanced glycation end-products regulate extracellular matrix-adipocyte metabolic crosstalk in diabetes. Sci Rep 9:19748 (2019). PubMed: 31875018