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Mouse IgG2b, kappa monoclonal [MG2b-57] - Isotype control (ab18421)

Q&A (1)References (4)

Conjugates logo Related conjugates and formulations

FITC

Overview

  • Product name

    Mouse IgG2b, kappa monoclonal [MG2b-57] - Isotype control
    See all Mouse isotype controls

  • Specificity

    The MG2b-57 immunoglobulin (ab18421) reacts with KLH. The MG2b-57 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

  • Tested applications

    Suitable for: IP, WB, IHC-Fr, IHC-P, Flow Cytmore details

  • Immunogen

    Trinitrophenol + KLH

  • General notes

    ab18421 MG2b-57 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    The immunoglobulin was purified by affinity chromatography.

  • Isotype control notes

    ab18421 MG2b-57 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

  • Clonality

    Monoclonal

  • Clone number

    MG2b-57

  • Isotype

    IgG2b

  • Light chain type

    kappa

  • Research areas

  • Alternative names

    • Mouse Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Surface or intracellular staining.
Notes
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Surface or intracellular staining.

References (4)

Publishing research using ab18421? Please let us know so that we can cite the reference in this datasheet.

ab18421 has been referenced in 4 publications.

  • Liu C  et al. The mechanosensitive lncRNA Neat1 promotes osteoblast function through paraspeckle-dependent Smurf1 mRNA retention. Bone Res 10:18 (2022). PubMed: 35210394
  • Zhao X  et al. Human Intestinal Organoids Recapitulate Enteric Infections of Enterovirus and Coronavirus. Stem Cell Reports 16:493-504 (2021). PubMed: 33626333
  • D'Onofrio N  et al. MicroRNA-33 and SIRT1 influence the coronary thrombus burden in hyperglycemic STEMI patients. J Cell Physiol 235:1438-1452 (2020). PubMed: 31294459
  • Mercado-Matos J  et al. Differential involvement of the microtubule cytoskeleton in insulin receptor substrate 1 (IRS-1) and IRS-2 signaling to AKT determines the response to microtubule disruption in breast carcinoma cells. J Biol Chem 292:7806-7816 (2017). PubMed: 28320862

Customer reviews and Q&As

Q&A
Submit a question

Question

Hi,   I purchased a pair of antibodies to detect mouse IgG2b by sandwich assays. The antibody that I have for the capture probe is ab11566 (Rat monoclonal [LO-MG2b-1]). And I have ab99640 (Rat monoclonal [LO-MG2b-2], biotin) as the detection probe. As proof of concept, I’d like to detect known isotype control samples using the pair of antibodies that I have. Now, I’m interested in ab18421 ([MG2b-57]) and ab18469 ([MPC-11]). Which one do you recommend? Are both isotype control samples recognized by the antibodies that I have? Another question I’d like to ask is what is the meaning of clone numbers like MG2b-1 and MG2b-2? Can I know the epitope information from these numbers? Thank you.

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Answer

Thank you for your enquiry.


Both you capture and detection antibodies should recognize these isotype controls. However, as these products have not been tested in sandwich ELISA we do not know whether the capture antibody will recognize the same site or otherwise interfere with the detection antibody. Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. As these products have not been tested in this application and they have not been tested against one anotherI cannot recommend one isotype control over the other.

Regarding the clone numbers, these are created by the originator the antibody for identification purposes. The epitopes of these products have not been mapped to our knowledge.



I look forward to hearing from you. Should you have any further questions please do not hesitate to ask

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