Mouse IgG2b, kappa monoclonal [MG2b-57] - Isotype Control (Low endotoxin, azide free) (ab18428)
Related conjugates and formulations
Overview
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Product name
Mouse IgG2b, kappa monoclonal [MG2b-57] - Isotype Control (Low endotoxin, azide free)
See all Mouse isotype controls -
Tested applications
Suitable for: ICC/IF, IP, WB, IHC-Fr, Functional Studies, IHC-P, Flow Cytmore details -
Immunogen
Trinitrophenol + KLH
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General notes
ab18428 solution contains no preservative; handle under aseptic conditions.
ab18428 is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, bioassay, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. ab18428 reacts with KLH and the MG2b-57 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS
0.2 µm filter sterilized -
Carrier free
Yes -
Concentration information loading...
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Purity
Immunogen affinity purified -
Isotype control notes
ab18428 is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, bioassay, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. ab18428 reacts with KLH and the MG2b-57 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. -
Clonality
Monoclonal -
Clone number
MG2b-57 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
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Alternative names
- Mouse Isotype Control
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration. PubMed: 28024734
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Functional Studies |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
Suitable for surface or intracellular staining. |
Notes |
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ICC/IF
Use at an assay dependent concentration. PubMed: 28024734 |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. Suitable for surface or intracellular staining. |
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab18428 has been referenced in 3 publications.
- Sellgren CM et al. Increased synapse elimination by microglia in schizophrenia patient-derived models of synaptic pruning. Nat Neurosci 22:374-385 (2019). PubMed: 30718903
- Lenhard SC et al. In Vivo Imaging of Small Molecular Weight Peptides for Targeted Renal Drug Delivery: A Study in Normal and Polycystic Kidney Diseased Mice. J Pharmacol Exp Ther 370:786-795 (2019). PubMed: 30936291
- Wang X et al. Lyn kinase represses mucus hypersecretion by regulating IL-13-induced endoplasmic reticulum stress in asthma. EBioMedicine 15:137-149 (2017). ICC/IF . PubMed: 28024734