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Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype control (ab18469)

Q&A (3)

Conjugates logo Related conjugates and formulations

Biotin FITC PE/Cy5® PerCP PerCP/Cy5.5®

Overview

  • Product name

    Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype control
    See all Mouse isotype controls

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, Flow Cyt, IPmore details

  • Immunogen

    Trinitrophenol and KLH.

  • General notes

    Isotype control has been screened on a variety of resting, activated, live, and fixed rat and human tissues.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    MPC-11

  • Isotype

    IgG2b

  • Light chain type

    kappa

  • Research areas

  • Alternative names

    • Mouse Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
WB
Flow Cyt
IP
Notes
Application notes
Flow Cyt: Use at an assay dependent dilution for surface and intracellular staining.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

References (0)

Publishing research using ab18469? Please let us know so that we can cite the reference in this datasheet.

ab18469 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Q&A
Submit a question

1-3 of 3 Q&A

Question

I have found two mouse IgG2b isotype controls which I think are suitable for my IHC-P experiments on human endobronchial biopsies, but I have a question about them:
-ab18421 clone MG2b-57 - immunogen used is Trinitrophenol + KLH, and the AB is said to react with KLH.
-ab18469 clone MPC-11 - immunogen used is also Trinitrophenol + KLH. My question is if this AB reacts with KLH like clone ab18421. In this, case do they react with different epitopes?

Thank you for your assistance,

Read More
Answer

Thank you for contacting Abcam.

We have not tested in-house reactivity of ab18469 against KLH, so I cannot say for certain whether the antibody will or will not bind to the KLH tag.

We have also not epitope mapped these antibodies, so we cannot say exactly where they bind, but I would suspect that they bind to different regions.

Please let me know if there is anything else I can help you with.

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Question

Hi,   I purchased a pair of antibodies to detect mouse IgG2b by sandwich assays. The antibody that I have for the capture probe is ab11566 (Rat monoclonal [LO-MG2b-1]). And I have ab99640 (Rat monoclonal [LO-MG2b-2], biotin) as the detection probe. As proof of concept, I’d like to detect known isotype control samples using the pair of antibodies that I have. Now, I’m interested in ab18421 ([MG2b-57]) and ab18469 ([MPC-11]). Which one do you recommend? Are both isotype control samples recognized by the antibodies that I have? Another question I’d like to ask is what is the meaning of clone numbers like MG2b-1 and MG2b-2? Can I know the epitope information from these numbers? Thank you.

Read More
Answer

Thank you for your enquiry.


Both you capture and detection antibodies should recognize these isotype controls. However, as these products have not been tested in sandwich ELISA we do not know whether the capture antibody will recognize the same site or otherwise interfere with the detection antibody. Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. As these products have not been tested in this application and they have not been tested against one anotherI cannot recommend one isotype control over the other.

Regarding the clone numbers, these are created by the originator the antibody for identification purposes. The epitopes of these products have not been mapped to our knowledge.



I look forward to hearing from you. Should you have any further questions please do not hesitate to ask

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Question

Dear Sir or Madam:

I would like to have the original reference of the isolation of hybridoma clone IgG2b MPC-11 (cat#ab18469).

Read More
Answer

Thank you for contacting us.

The MPC-11 myeloma is desribed in the following paper:

J Exp Med. 1970 Mar 1;131(3):515-41.
Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. Laskov R, Scharff MD. PMID:4189836


http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138817/pdf/515.pdf


My understanding is that the MPC-11 isotype control is not in fact a hybridoma but is simply a myeloma that secretes full-length IgG2b with no known specificity.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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