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Recombinant

Recombinant Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
  • Immunocytochemistry/ Immunofluorescence - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
  • Flow Cytometry - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
  • Immunocytochemistry/ Immunofluorescence - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
  • Immunocytochemistry/ Immunofluorescence - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
  • Flow Cytometry - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
  • Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)

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Carrier Free

Overview

  • Product name

    Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IFmore details

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    R311-MouseIgG2b

  • Isotype

    IgG2b

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab281590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/500.
ICC/IF
1/20.
Notes
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/500.
ICC/IF
1/20.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Mouse IgG2b with ab281590 at 1/200 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Negative control: No staining on human tonsil. Counterstained with Hematoxylin. 

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

  • Immunocytochemistry/ Immunofluorescence - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
    Immunocytochemistry/ Immunofluorescence - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling Mouse IgG2b with ab281590 at 1/20 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Confocal image showing no staining in HeLa cells.

    Negative control 1: ab281590 at a 1/20 dilution followed by ab150113 at a 1/1000 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150080 at a 1/1000 dilution.

  • Flow Cytometry - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
    Flow Cytometry - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Mouse IgG2b with ab281590 at 1/500 dilution (0.1µg) (Red) compared with a Mouse (E7Q5L) mAb IgG2b Isotype Control (Black) and unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
    Immunocytochemistry/ Immunofluorescence - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labelling Mouse IgG2b with ab281590 at 1/20 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Confocal image showing no staining in NIH/3T3 cells.

    Negative control 1: ab281590 at a 1/20 dilution followed by ab150113 at a 1/1000 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150080 at a 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
    Immunocytochemistry/ Immunofluorescence - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 cells labelling Mouse IgG2b with ab281590 at 1/20 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Confocal image showing no staining in PC-12 cells.

    Negative control 1: ab281590 at a 1/20 dilution followed by ab150113 at a 1/1000 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150080 at a 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Mouse IgG2b with ab281590 at 1/200 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Negative control: No staining on rat spleen. Counterstained with Hematoxylin. 

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

  • Flow Cytometry - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
    Flow Cytometry - Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast, Left) / PC-12 (Rat adrenal gland pheochromocytoma, Right) cells labelling Mouse IgG2b with ab281590 at 1/500 dilution (0.1µg) (Red) compared with a Mouse (E7Q5L) mAb IgG2b Isotype Control (Black) and unlabelled control (cells without incubation with primary antibody and secondary antibody)(Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

  • Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)
    Mouse IgG2b monoclonal [R311-MouseIgG2b] - Isotype control (ab281590)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab281590? Please let us know so that we can cite the reference in this datasheet.

ab281590 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Q&A
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