Recombinant Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)
![Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)](https://www.abcam.com/ps/products/280/ab280756/Images/ab280756-454733-antimouseigg2cantibodyr313mouseigg2cimmunocytochemistrymolt4human1.jpg "Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)")
Related conjugates and formulations
Overview
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Product name
Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IFmore details
Unsuitable for: IHC-P -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
R313-MouseIgG2c -
Isotype
IgG2c
Associated products
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Alternative Versions
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Compatible Secondaries
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Dyes/Markers
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab280756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/1000.
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| ICC/IF |
1/80.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/1000. |
|
ICC/IF
1/80. |
Images
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Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in MOLT-4 cells.
Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)](https://www.abcam.com/ps/products/280/ab280756/Images/ab280756-454736-antimouseigg2cantibodyr313mouseigg2cflowcytometrymolt4human.jpg)
Flow Cytometry (Intracellular) - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)](https://www.abcam.com/ps/products/280/ab280756/Images/ab280756-454739-antimouseigg2cantibodyr313mouseigg2cimmunocytochemistrynih3t3mouse1.jpg)
Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in NIH/3T3 cells.
Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)](https://www.abcam.com/ps/products/280/ab280756/Images/ab280756-454740-antimouseigg2cantibodyr313mouseigg2cflowcytometrynih3t3mouse.jpg)
Flow Cytometry (Intracellular) - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast, Left) / PC-12 (Rat adrenal gland pheochromocytoma, Right) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)](https://www.abcam.com/ps/products/280/ab280756/Images/ab280756-454741-antimouseigg2cantibodyr313mouseigg2cimmunocytochemistrypc12rat1.jpg)
Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in PC-12 cells.
Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
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![Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)](https://www.abcam.com/ps/products/280/ab280756/Images/ab280756-454735-benefitsofrecombinantantibodies.png)
Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control (ab280756)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab280756 has not yet been referenced specifically in any publications.