Recombinant Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)
![Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)](https://www.abcam.com/ps/products/280/ab280757/Images/ab280757-454748-antimouseigg2cantibodyr313mouseigg2cimmunocytochemistrymolt4human1.jpg "Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)")
Related conjugates and formulations
Overview
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Product name
Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IFmore details
Unsuitable for: IHC-P -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
ab280757 is the carrier-free version of ab280756. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab280757 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
R313-MouseIgG2c -
Isotype
IgG2c
Associated products
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Alternative Versions
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Compatible Secondaries
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Dyes/Markers
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab280757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
Images
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Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)
This image was produced using ab280756, the same clone but in a different formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in MOLT-4 cells.
Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)](https://www.abcam.com/ps/products/280/ab280757/Images/ab280757-454747-antimouseigg2cantibodyr313mouseigg2cflowcytometrymolt4human.jpg)
Flow Cytometry (Intracellular) - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)This image was produced using ab280756, the same clone but in a different formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)](https://www.abcam.com/ps/products/280/ab280757/Images/ab280757-454746-antimouseigg2cantibodyr313mouseigg2cimmunocytochemistrynih3t3mouse1.jpg)
Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)This image was produced using ab280756, the same clone but in a different formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in NIH/3T3 cells.
Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)](https://www.abcam.com/ps/products/280/ab280757/Images/ab280757-454745-antimouseigg2cantibodyr313mouseigg2cflowcytometrynih3t3mouse.jpg)
Flow Cytometry (Intracellular) - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)This image was produced using ab280756, the same clone but in a different formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast, Left) / PC-12 (Rat adrenal gland pheochromocytoma, Right) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)](https://www.abcam.com/ps/products/280/ab280757/Images/ab280757-454744-antimouseigg2cantibodyr313mouseigg2cimmunocytochemistrypc12rat1.jpg)
Immunocytochemistry/ Immunofluorescence - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)This image was produced using ab280756, the same clone but in a different formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NPC-12 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in PC-12 cells.
Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
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![Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)](https://www.abcam.com/ps/products/280/ab280757/Images/ab280757-454743-benefitsofrecombinantantibodies.png)
Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab280757 has not yet been referenced specifically in any publications.
