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Mouse IgG3, kappa monoclonal [MG3-35] - Isotype Control (ab18394)

Q&A (5)References (4)

Conjugates logo Related conjugates and formulations

Biotin

Overview

  • Product name

    Mouse IgG3, kappa monoclonal [MG3-35] - Isotype Control
    See all Mouse isotype controls

  • Tested applications

    Suitable for: IP, WB, IHC-Fr, IHC-P, Flow Cytmore details

  • Immunogen

    Full length protein corresponding to Mouse conjugated to keyhole limpet haemocyanin. Trinitrophenol

  • General notes

    ab18394 reacts with KLH. It was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Isotype control notes

    Ab18394 reacts with KLH. It was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

  • Clonality

    Monoclonal

  • Clone number

    MG3-35

  • Isotype

    IgG3

  • Light chain type

    kappa

  • Research areas

  • Alternative names

    • Mouse Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.
Notes
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

References (4)

Publishing research using ab18394? Please let us know so that we can cite the reference in this datasheet.

ab18394 has been referenced in 4 publications.

  • Binch A  et al. Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues. JOR Spine 3:e1098 (2020). PubMed: 33015573
  • Kolanko E  et al. Increased immunomodulatory capacity of human amniotic cells after activation by pro-inflammatory chemokines. Eur J Pharmacol 859:172545 (2019). PubMed: 31319066
  • Harlow ML  et al. Trabectedin Inhibits EWS-FLI1 and Evicts SWI/SNF from Chromatin in a Schedule-dependent Manner. Clin Cancer Res 25:3417-3429 (2019). PubMed: 30723142
  • Mitchell J & Sullam PM Streptococcus mitis phage-encoded adhesins mediate attachment to {alpha}2-8-linked sialic acid residues on platelet membrane gangliosides. Infect Immun 77:3485-90 (2009). PubMed: 19506011

Customer reviews and Q&As

Q&A
Submit a question

1-5 of 5 Q&A

Question

We recently received a shipment of ab18394 and need to assign an expiration date to the raw material.

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Answer

Thank you for contacting Abcam.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least 6 months from the date of purchase when stored correctly.

For ab18394, we recommened that you store the product at 4C

For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols).

I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Question

I need a Certificate of Analysis for both lots of material shipped to me today.

Please e-mail them as soon as possible so we can process the shipment.

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Answer

Thank you for contacting Abcam regarding ab18394.


Attached please find the document you requested.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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Question

Thank you for your reply.  First of all, kindly advise the pattern of the run that the customer should observe with the isotype control.   Secondly, please read the attached correspondence regarding the compatibility of ab18394 with IP and Invitrogen's Dynabeads protein A #100.01D. Please clarify.   Thanks in advance for your assistance and reply.  

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Answer

Thank you for contacting us.

I did read over the specifications for dynabeads and I did not realize that they claim to have strong binding with IgG3 as most protein A beads have a weak binding with that particular isotype. However, because the primary antibody is IgG2b a correct isotype control will be anti-Mouse IgG2b.

Any isotype control should exhibit a typical banding of the heavy chain at ˜55kDa and of the light chain at ˜25kDa if the antibody has been reduced/denatured. Would it be possible to send the full membrane image with ladder as I was wondering if the light chain is present?

As always, this product is covered by our Abpromise guarantee.
If we cannot remedy this issue and this is a product that you have purchased within the last six months, we will replace or refund it under our Abpromise guarantee, as you are using it according to specifications listed on our datasheet.

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Question

Description of the problem: in isotype control lane of the IP performed there is no typical strong IgG heavy chain band observed in the experiment lane   Sample Nuclear extract digested with MNase Sample preparation incubation of sample with IP Ab’ ON 4 deg C, rotation 4 h with protein A dynal beads to form Matrix-antibody-antigen complex, extensive washes with RIPA buffer.   Matrix used to allow precipitation of antibody-antigen complex  invitrogen™, Dynabeads® protein A Detergent present in your system 0.7% DOC, 1% NP-40, 0.1% SDS Electrophoresis/Gel conditions SDS-PAGE 4-20% gradient gel Primary Antibody custom Mouse α hnRNP (all isoforms) Expected size, 35-40 kd. TBST 1% (sodime azide) BSA 4deg ON, 3 20 min wash with TBST   Secondary Antibody Jackson Peroxidase-conjugated AffiniPure Goat α mouse IgG (H+L) (1:40000) in 5% skim milk, 3 20 min wash with TBST Detection method Autoradiography                                                                                

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Answer

Thank you for contacting us and thank you for providing the protocol and image.
After reviewing the data presented I would like to suggest a few changes that may help improve your results.

Mouse IgG3 does not bind well to beads for IP. To overcome this we recommend that when using an IgG3 antibody that you incubate for a longer period and use higher concentration. As your primary antibody is IgG2b, which binds more strongly to protein A beads, a correct isotype control will be anti-Mouse IgG2b. We do offer this product, ab18421. I have placed the link below.

https://www.abcam.com/Mouse-IgG2b-kappa-monoclonal-MG2b-57-Isotype-control-ab18421.html


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Hi, Do you know the IgG concentration of isotypektr. Mouse IgG3,kappa, ab 18394? Best regards  

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Answer

Thank you for contacting us. The concentration of this product is 0.500 mg/ml. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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