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Recombinant

Recombinant Anti-Mouse IgM antibody [RM109] (ab190369)

Submit a review Submit a question References (3)
ELISA - Anti-Mouse IgM antibody [RM109] (ab190369)
  • ELISA - Anti-Mouse IgM antibody [RM109] (ab190369)
  • Anti-Mouse IgM antibody [RM109] (ab190369)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM109] to Mouse IgM
  • Suitable for: ELISA, ICC, IHC-P, IP, Flow Cyt
  • Reacts with: Mouse, Recombinant fragment

Overview

  • Product name

    Anti-Mouse IgM antibody [RM109]

  • Description

    Rabbit monoclonal [RM109] to Mouse IgM

  • Host species

    Rabbit

  • Specificity

    This antibody reacts to mouse IgM. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The Fc region of RM109 has been engineered to eliminate the binding of Fc receptor.

  • Tested applications

    Suitable for: ELISA, ICC, IHC-P, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Recombinant fragment

  • Immunogen

    Full length protein corresponding to Mouse Mouse IgM.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    ab190369 was purified from an animal origin–free and protein-free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RM109

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab190369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use a concentration of 0.005 - 0.2 µg/ml.
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
ELISA
Use a concentration of 0.005 - 0.2 µg/ml.
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Relevance

    IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. There are two isoforms.

  • Cellular localization

    Plasma membrane and Secreted

  • Database links

    • Alternative names

      • heavy chain of IgM antibody
      • Ig mu chain C region antibody
      • Ig mu chain C region secreted form antibody
      • Igh 6 antibody
      • Igh-6 antibody
      • IGHM antibody
      • Igm antibody
      • immunoglobulin heavy chain antibody
      see all

    Images

    • ELISA - Anti-Mouse IgM antibody [RM109] (ab190369)
      ELISA - Anti-Mouse IgM antibody [RM109] (ab190369)

      A titer ELISA of mouse IgM. The plate was coated with different amounts of mouse IgM. A serial dilution of ab190369 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    • ELISA - Anti-Mouse IgM antibody [RM109] (ab190369)
      ELISA - Anti-Mouse IgM antibody [RM109] (ab190369)

      ELISA of mouse immunoglobulins shows ab190369 reacted to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab190369 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    • Anti-Mouse IgM antibody [RM109] (ab190369)
      Anti-Mouse IgM antibody [RM109] (ab190369)

    References (3)

    Publishing research using ab190369? Please let us know so that we can cite the reference in this datasheet.

    ab190369 has been referenced in 3 publications.

    • Liu F  et al. Enrichment differentiation of human induced pluripotent stem cells into sinoatrial node-like cells by combined modulation of BMP, FGF, and RA signaling pathways. Stem Cell Res Ther 11:284 (2020). PubMed: 32678003
    • Xie A  et al. T follicular helper and memory cell responses and the mTOR pathway in murine heart transplantation. J Heart Lung Transplant 39:134-144 (2020). PubMed: 31831210
    • Hu X  et al. Respiratory Syncytial Virus Exacerbates Kidney Damages in IgA Nephropathy Mice via the C5a-C5aR1 Axis Orchestrating Th17 Cell Responses. Front Cell Infect Microbiol 9:151 (2019). PubMed: 31134161

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