Recombinant Anti-Mouse IgM antibody [RM109] (ab190369)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM109] to Mouse IgM
- Suitable for: ELISA, ICC, IHC-P, IP, Flow Cyt
- Reacts with: Mouse, Recombinant fragment
Overview
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Product name
Anti-Mouse IgM antibody [RM109] -
Description
Rabbit monoclonal [RM109] to Mouse IgM -
Host species
Rabbit -
Specificity
This antibody reacts to mouse IgM. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The Fc region of RM109 has been engineered to eliminate the binding of Fc receptor. -
Tested applications
Suitable for: ELISA, ICC, IHC-P, IP, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Recombinant fragment -
Immunogen
Full length protein corresponding to Mouse Mouse IgM.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab190369 was purified from an animal origin–free and protein-free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM109 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use a concentration of 0.005 - 0.2 µg/ml.
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ICC |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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ELISA
Use a concentration of 0.005 - 0.2 µg/ml. |
ICC
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. There are two isoforms. -
Cellular localization
Plasma membrane and Secreted -
Database links
- Entrez Gene: 16019 Mouse
- SwissProt: P01872 Mouse
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Alternative names
- heavy chain of IgM antibody
- Ig mu chain C region antibody
- Ig mu chain C region secreted form antibody
see all
Images
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A titer ELISA of mouse IgM. The plate was coated with different amounts of mouse IgM. A serial dilution of ab190369 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA of mouse immunoglobulins shows ab190369 reacted to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab190369 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab190369 has been referenced in 3 publications.
- Liu F et al. Enrichment differentiation of human induced pluripotent stem cells into sinoatrial node-like cells by combined modulation of BMP, FGF, and RA signaling pathways. Stem Cell Res Ther 11:284 (2020). PubMed: 32678003
- Xie A et al. T follicular helper and memory cell responses and the mTOR pathway in murine heart transplantation. J Heart Lung Transplant 39:134-144 (2020). PubMed: 31831210
- Hu X et al. Respiratory Syncytial Virus Exacerbates Kidney Damages in IgA Nephropathy Mice via the C5a-C5aR1 Axis Orchestrating Th17 Cell Responses. Front Cell Infect Microbiol 9:151 (2019). PubMed: 31134161