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RecombinantRabMAb

Recombinant Anti-Mov10 antibody [EPR14478] (ab189919)

Submit a review Submit a question References (1)
Western blot - Anti-Mov10 antibody [EPR14478] (ab189919)
  • Western blot - Anti-Mov10 antibody [EPR14478] (ab189919)
  • Western blot - Anti-Mov10 antibody [EPR14478] (ab189919)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mov10 antibody [EPR14478] (ab189919)
  • Anti-Mov10 antibody [EPR14478] (ab189919)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14478] to Mov10
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Mov10 antibody [EPR14478]
    See all Mov10 primary antibodies

  • Description

    Rabbit monoclonal [EPR14478] to Mov10

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver, 293, HeLa , HepG2, C6, NIH/3T3 and Raw264.7 lysates; Human pancreas tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab189919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/2000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    MOV10 may be an helicase with an important function in development and/or control of cell proliferation. RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs). They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs. Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA. Ago1- and Ago2-containing complexes have been purified from human cells, resulting in the discovery of novel factors such as the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage.

  • Cellular localization

    Cytoplasmic

  • Database links

    • Alternative names

      • DKFZp667O1423 antibody
      • FLJ32791 antibody
      • fSAP113 antibody
      • Functional spliceosome-associated protein 113 antibody
      • gb110 antibody
      • MGC2948 antibody
      • Moloney leukemia virus 10 protein antibody
      • MOV 10 antibody
      • Mov10 (Moloney leukemia virus 10, mouse) homolog antibody
      • Mov10 (Moloney leukemia virus 10,) homolog antibody
      • Mov10, Moloney leukemia virus 10, homolog (mouse) antibody
      • Mov10, Moloney leukemia virus 10, homolog antibody
      • Putative helicase MOV10 antibody
      see all

    Images

    • Western blot - Anti-Mov10 antibody [EPR14478] (ab189919)
      Western blot - Anti-Mov10 antibody [EPR14478] (ab189919)
      All lanes : Anti-Mov10 antibody [EPR14478] (ab189919) at 1/5000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : 293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 114 kDa
      Observed band size: 114 kDa

    • Western blot - Anti-Mov10 antibody [EPR14478] (ab189919)
      Western blot - Anti-Mov10 antibody [EPR14478] (ab189919)
      All lanes : Anti-Mov10 antibody [EPR14478] (ab189919) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 114 kDa
      Observed band size: 114 kDa

    • Western blot - Anti-Mov10 antibody [EPR14478] (ab189919)
      Western blot - Anti-Mov10 antibody [EPR14478] (ab189919)
      All lanes : Anti-Mov10 antibody [EPR14478] (ab189919) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : NIH/3T3 cell lysate
      Lane 3 : Raw264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 114 kDa
      Observed band size: 114 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mov10 antibody [EPR14478] (ab189919)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mov10 antibody [EPR14478] (ab189919)

      Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Mov10 with ab189919 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-Mov10 antibody [EPR14478] (ab189919)
      Anti-Mov10 antibody [EPR14478] (ab189919)

    References (1)

    Publishing research using ab189919? Please let us know so that we can cite the reference in this datasheet.

    ab189919 has been referenced in 1 publication.

    • Martin-Jaular L  et al. Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection. EMBO J 40:e105492 (2021). PubMed: 33709510

    Customer reviews and Q&As

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