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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)

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Immunoprecipitation - Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)
  • Western blot - Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)
  • Western blot - Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)
  • Western blot - Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)
  • Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12457] to MRas - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-MRas antibody [EPR12457] - BSA and Azide free
    See all MRas primary antibodies

  • Description

    Rabbit monoclonal [EPR12457] to MRas - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: mouse brain, rate brain; human brain; HeLa cells IP: human heart lysate

  • General notes

    ab240194 is the carrier-free version of ab176570.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab240194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
IP
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
IP
Use at an assay dependent concentration.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

  • Function

    May serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway.

  • Tissue specificity

    Expression highly restricted to the brain and heart.

  • Sequence similarities

    Belongs to the small GTPase superfamily. Ras family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • FLJ42964 antibody
    • M ras antibody
    • M-ras antibody
    • Mras antibody
    • Muscle and microspikes Ras antibody
    • Muscle RAS oncogene homolog antibody
    • Muscle Ras oncogene homologue antibody
    • Muscle Ras viral oncogene homolog antibody
    • R ras3 antibody
    • R-ras3 antibody
    • Ras related protein MRas antibody
    • Ras related protein RRas3 antibody
    • Ras-related protein M-Ras antibody
    • Ras-related protein R-Ras3 antibody
    • RASM_HUMAN antibody
    • Related Ras viral oncogene homolog 3 antibody
    • RRas3 antibody
    • XRas antibody
    see all

Images

  • Immunoprecipitation - Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)
    Immunoprecipitation - Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)

    This data was developed using ab176570, the same antibody clone in a different buffer formulation.
    Purified ab176570 at 1/20 dilution (1µg) immunoprecipitating MRas in Human heart lysate.
    Lane 1 (input): Human heart lysate 10µg
    Lane 2 (+): ab176570 + Human heart lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab176570 in Human heart lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 24 kDa

  • Western blot - Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)
    Western blot - Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)
    Lane 1 : Anti-MRas antibody [EPR12457] (ab176570) at 1/5000 dilution (Purified)
    Lane 2 : Anti-MRas antibody [EPR12457] (ab176570) at 1/5000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 24 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab176570).

     

  • Western blot - Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)
    Western blot - Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)
    Anti-MRas antibody [EPR12457] (ab176570) at 1/1000 dilution (Purified) + Human brain lysate

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 24 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab176570).

     

  • Western blot - Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)
    Western blot - Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)
    All lanes : Anti-MRas antibody [EPR12457] (ab176570) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : MRAS knockout HeLa cell lysate
    Lane 3 : Human brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 24 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab176570).

    Lanes 1-3: Merged signal (red and green). Green - ab176570 observed at 26 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab176570 Anti-MRas antibody [EPR12457] was shown to specifically react with MRas in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265510 (knockout cell lysate ab257541) was used. Wild-type and MRas knockout samples were subjected to SDS-PAGE. ab176570 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)
    Anti-MRas antibody [EPR12457] - BSA and Azide free (ab240194)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab240194? Please let us know so that we can cite the reference in this datasheet.

ab240194 has not yet been referenced specifically in any publications.

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