Recombinant Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)
![Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)](https://www.abcam.com/ps/products/180/ab180960/Images/ab180960-352598-anti-mrp1-antibody-epr46582-western-blot-knockout-hela-human-lysates.jpg "Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4658(2)] to MRP1 - C-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MRP1 antibody [EPR4658(2)] - C-terminal
See all MRP1 primary antibodies -
Description
Rabbit monoclonal [EPR4658(2)] to MRP1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A431: HeLa and A549 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4658(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 171 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 171 kDa. |
Target
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Function
Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency. -
Tissue specificity
Lung, testis and peripheral blood mononuclear cells. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4363 Human
- Omim: 158343 Human
- SwissProt: P33527 Human
- Unigene: 391464 Human
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Alternative names
- ABC 29 antibody
- ABC29 antibody
- ABCC 1 antibody
see all
Images
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Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)All lanes : Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960) at 1/1000 dilution
Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : ABCC1 knockout A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 171 kDaLanes 1 - 2: Merged signal (red and green). Green - ab180960 observed at 170 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab180960 was shown to specifically react with ABCC1 in wild-type A549 cells as signal was lost in ABCC1 knockout cells. Wild-type and ABCC1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab180960 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging
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![Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)](https://www.abcam.com/ps/products/180/ab180960/Images/ab180960-338635-anti-mrp1-antibody-epr46582-c-terminal-western-blot.png)
Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)All lanes : Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate (boiled)
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 171 kDa
Exposure time: 20 secondsBlocking and dilution buffer: 5% NFDM/TBST.
If there is no ideal result on boiled sample, we suggest not to boil sample before loading onto the gel.
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![Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)](https://www.abcam.com/ps/products/180/ab180960/Images/ab180960-212267-anti-mrp1-antibody-epr46582-c-terminal-western-blot.jpg)
Western blot - Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960) at 1/5000 dilution + A549 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 171 kDa -
![Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)](https://www.abcam.com/ps/products/180/ab180960/Images/ab180960-6-benefits-of-recombinant-antibodies.png)
Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (10)
ab180960 has been referenced in 10 publications.
- Low FG et al. Roles of ABCC1 and ABCC4 in Proliferation and Migration of Breast Cancer Cell Lines. Int J Mol Sci 21:N/A (2020). PubMed: 33081264
- Wang C et al. Silencing long non-coding RNA XIST suppresses drug resistance in acute myeloid leukemia through down-regulation of MYC by elevating microRNA-29a expression. Mol Med 26:114 (2020). PubMed: 33228517
- Li Y et al. Involvement of the PI3K/Akt/Nrf2 Signaling Pathway in Resveratrol-Mediated Reversal of Drug Resistance in HL-60/ADR Cells. Nutr Cancer 71:1007-1018 (2019). PubMed: 31032633
- Wang X et al. Fatty acid receptor GPR120 promotes breast cancer chemoresistance by upregulating ABC transporters expression and fatty acid synthesis. EBioMedicine 40:251-262 (2019). PubMed: 30738829
- Wang B et al. Oridonin overcomes the gemcitabine resistant PANC-1/Gem cells by regulating GST pi and LRP/1 ERK/JNK signalling. Onco Targets Ther 12:5751-5765 (2019). PubMed: 31410021
- Mao BD et al. LINC00511 knockdown prevents cervical cancer cell proliferation and reduces resistance to paclitaxel. J Biosci 44:N/A (2019). PubMed: 31180057
- Li S et al. Silencing of Long Non-coding RNA SMAD5-AS1 Reverses Epithelial Mesenchymal Transition in Nasopharyngeal Carcinoma via microRNA-195-Dependent Inhibition of SMAD5. Front Oncol 9:1246 (2019). PubMed: 31921616
- Guo F et al. The action mechanism of lncRNA-HOTAIR on the drug resistance of non-small cell lung cancer by regulating Wnt signaling pathway. Exp Ther Med 15:4885-4889 (2018). PubMed: 29805510
- Song L et al. MicroRNA-340-5p modulates cisplatin resistance by targeting LPAATß in osteosarcoma. Braz J Med Biol Res 50:e6359 (2017). WB . PubMed: 28443990
- Liu Z & Zhang H LncRNA plasmacytoma variant translocation 1 is an oncogene in bladder urothelial carcinoma. Oncotarget 8:64273-64282 (2017). PubMed: 28969069