Anti-MTA1 antibody (ab226960)
Key features and details
- Rabbit polyclonal to MTA1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-MTA1 antibody
See all MTA1 primary antibodies -
Description
Rabbit polyclonal to MTA1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Positive control
- IHC-P: Human breast carcinoma tissue; Mouse ovary tissue; Rat hind brain tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/1000.
|
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
|
IHC-P
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
May be involved in the regulation of gene expression by covalent modification of histone proteins. Isoform Long is a corepressor of estrogen receptor (ER). Isoform Short binds to ER and sequesters it in the cytoplasm and enhances non-genomic responses of ER. -
Tissue specificity
Widely expressed. High expression in brain, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual. -
Sequence similarities
Contains 1 BAH domain.
Contains 1 ELM2 domain.
Contains 1 GATA-type zinc finger.
Contains 1 SANT domain. -
Developmental stage
Highly expressed in metastatic cells. -
Domain
Isoform Short contains a Leu-Arg-Ile-Leu-Leu motif (ER binding motif). -
Cellular localization
Cytoplasm and Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9112 Human
- Entrez Gene: 116870 Mouse
- Entrez Gene: 64520 Rat
- Omim: 603526 Human
- SwissProt: Q13330 Human
- SwissProt: Q8K4B0 Mouse
- SwissProt: Q62599 Rat
- Unigene: 525629 Human
see all -
Alternative names
- Metastasis associated 1 antibody
- Metastasis associated gene 1 antibody
- Metastasis associated gene 1 protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MTA1 antibody (ab226960)
4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for MTA1 (green) using ab226960 at 1/500 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA1 antibody (ab226960)Paraffin-embedded rat hind brain tissue stained for MTA1 using ab226960 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA1 antibody (ab226960)Paraffin-embedded mouse ovary tissue stained for MTA1 using ab226960 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA1 antibody (ab226960)Paraffin-embedded human breast carcinoma tissue stained for MTA1 using ab226960 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab226960 has been referenced in 1 publication.
- Wang Y et al. Myosin Heavy Chain 10 (MYH10) Gene Silencing Reduces Cell Migration and Invasion in the Glioma Cell Lines U251, T98G, and SHG44 by Inhibiting the Wnt/ß-Catenin Pathway. Med Sci Monit 24:9110-9119 (2018). PubMed: 30552850