Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)

Submit a review Submit a question References (1)
Western blot - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)
  • Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)
  • Flow Cytometry (Intracellular) - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)
  • Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8537(2)] to MTA2/PID
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-MTA2/PID antibody [EPR8537(2)]
    See all MTA2/PID primary antibodies

  • Description

    Rabbit monoclonal [EPR8537(2)] to MTA2/PID

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, Flow Cyt (Intra)more details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MCF7, HeLa and SW480 cell lysates. Paraffin-embedded Human kidney and Human colonic carcinoma tissue. HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab171073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/250 - 1/500.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended
WB
1/1000 - 1/5000. Predicted molecular weight: 75 kDa.
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
ICC/IF
1/250 - 1/500.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended
WB
1/1000 - 1/5000. Predicted molecular weight: 75 kDa.
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notes
Is unsuitable for IP.

Target

  • Function

    May be involved in the regulation of gene expression as repressor and activator. The repression might be related to covalent modification of histone proteins.

  • Tissue specificity

    Widely expressed.

  • Sequence similarities

    Contains 1 BAH domain.
    Contains 1 ELM2 domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 SANT domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • DKFZp686F2281 antibody
      • Mata1l1 antibody
      • Metastasis associated 1 family member 2 antibody
      • Metastasis associated 1 like 1 antibody
      • Metastasis associated gene 1 like 1 antibody
      • Metastasis associated gene family member 2 antibody
      • Metastasis associated protein 2 antibody
      • Metastasis associated protein MTA 2 antibody
      • Metastasis associated protein MTA2 antibody
      • Metastasis-associated 1-like 1 antibody
      • Metastasis-associated protein MTA2 antibody
      • Mmta2 antibody
      • MTA1 L1 protein antibody
      • MTA1-L1 protein antibody
      • MTA1L1 antibody
      • MTA2 antibody
      • MTA2_HUMAN antibody
      • p53 target protein in deacetylase complex antibody
      • PID antibody
      see all

    Images

    • Western blot - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)
      Western blot - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)
      All lanes : Anti-MTA2/PID antibody [EPR8537(2)] (ab171073) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 75 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)

      Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling MTA2/PID with ab171073 at 1/50 dilution. 

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MTA2/PID with ab171073 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)
      Immunocytochemistry/ Immunofluorescence - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)

      Immunofluorescent staining of HeLa cells labeling MTA2/PID with ab171073 at 1/250 dilution.

    • Flow Cytometry (Intracellular) - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)
      Flow Cytometry (Intracellular) - Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)

      Intracellular flow cytometric analysis of permeabilized Hela cells labeling MTA2/PID with ab171073 at 1/100 dilution (red)or a rabbit IgG negative (green). 

       

       

    • Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)
      Anti-MTA2/PID antibody [EPR8537(2)] (ab171073)

    References (1)

    Publishing research using ab171073? Please let us know so that we can cite the reference in this datasheet.

    ab171073 has been referenced in 1 publication.

    • Chen YS  et al. MTA2 as a Potential Biomarker and Its Involvement in Metastatic Progression of Human Renal Cancer by miR-133b Targeting MMP-9. Cancers (Basel) 11:N/A (2019). PubMed: 31771219

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab171073.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com